益气利水破血解毒小复方对NZB/WF1狼疮鼠肾脏水通道蛋白干预作用的研究  

Study on Aquaporin Expression in Renal Tissue of NZB/W F1 Mice with Intervention of Yiqi Lishui Poxue Jiedu Decoction

作　　者：王骁[1] 楼丹飞[2] 姚重华[2] 杨亚馗[3] 苏励[3] 曲环汝[3] 田雨[3] 薛轶燕[3] 覃光辉[3] 

机构地区：[1]上海中医药大学附属龙华医院风湿科,上海200030 [2]上海市中医医院老年科,上海200071 [3]上海中医药大学附属龙华医院,上海200030

出　　处：《四川中医》2013年第6期53-55,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:30472205);上海市市属博士学科点基金资助项目(编号:2006B09);2010年上海高校选拔培养优秀教师科研基金资助项目(编号:11102)

摘　　要：目的:探索益气利水破血解毒小复方干预对NZB/W F1狼疮鼠肾脏水通道蛋白AQP1和AQP3表达的影响,帮助进一步了解其作用机制和靶点。方法:将NZB/W F1狼疮鼠随机分为对照组(0.5ml/d生理盐水灌胃)、中药治疗组(益气利水破血解毒小复方0.5ml/d灌胃)和西药治疗组(骁悉水溶液0.5ml/d灌胃),分别于干预第0、2、4、6周留取尿液样本检测尿蛋白水平,干预8周后处死小鼠行肾脏免疫组化,半定量分析AQPl、AQP3表达水平。结果:中药组和西药组小鼠尿蛋白定量均明显低于对照组(P<0.01),两治疗组间未见统计差异(P>0.05);中药组和西药组小鼠肾组织AQP1表达量显著高于对照组(P<0.01),西药组AQP1表达量高于中药二组间存在统计学差异(p<0.05)。与对照组相比,中药组和西药组小鼠肾组织AQP3表达量显著较高,有统计学差异(p<0.01),西药组与中药组小鼠肾组织AQP3表达量未见统计学差异(p>0.05)。结论:益气利水破血解毒小复方干预可有效降低NZB/WF1狼疮鼠尿蛋白水平,并加强肾脏水通道蛋白AQP1和AQP3的表达。Objectives： To evaluate the influence of Yiqi Lishui Poxue Jiedu Decoction on aquaporin expression in renal tissue of NZB/W FI mice and tried to find out related mechanism and targets of the decoction. Methods： NZB/W F1 mice were allotted to control group （normal saline 0. 5ml/d po）, decoction group （Yiqi Lishui Poxue Jiedu Decoction 0. 5ml/d po） and cellcept group （cellcept solution 0. 5ml/d po）, 24h urine sample were taken on the 0, 2nd, 4th, 6thweek since intervention begun to test the urine protein level. Mice kidneys were harvested on the 8th week of intervention, with the immunohistochemistry stain, AQPI and AQP3 expression in renal tissue were estimated. Results： The urine protein level of both decoction group and cellcept group were significantly lower than the control group （P〈0. 01 ）, while no statistical difference was found between the two groups （P〉 0. 05）. AQP1 expression in renal tissue of both decoction group and cellcept group were higher than the control group （P〈0. O1 ）, and the cellcept group had a higher level of AQP1 than decoction group （P〈0. 05）. AQP3 expression in renal tissue of both decoction group and cellcept group were higher than the control group （ P〈0. 01）, while no statistical difference was found between the two groups （P〉0. 05）. Conclusion： The Yiqi Lishui Poxue Jiedu Decoction may relieve the protein urine and enhance the expression of both AQP1 and AQP3 in the renal tissue of NZB/W F1 mice.

关 键 词：NZB WF1 狼疮鼠 肾脏 水通道蛋白 

分 类 号：R285.5[医药卫生—中药学]