基因芯片技术阐释虚寒证与虚热证大鼠“精气夺则虚”的分子机制  

Explanation on the Molecular Mechanism of "Lack of Vital Essence Resulting in Deficiency Syndrome"of Asthenia Cold Syndrome and Asthenia Hot Syndrome Rats

作　　者：韩冰冰[1] 王世军[2] 

机构地区：[1]山东中医药大学基础医学院,山东济南250355 [2]山东中医药大学国际教育学院,山东济南250355

出　　处：《四川中医》2013年第6期60-63,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)课题资助(编号:2007CB512601;基于四性的中药性-效-物质关系研究);科技部973项目子课题(编号:2013CB531803)

摘　　要：目的:采用基因芯片技术阐释虚寒证与虚热证大鼠"精气夺则虚"的共同分子机制。方法:使用中药复方建立虚寒证、虚热证大鼠模型,基因芯片检测大鼠肝脏基因表达,筛选差异表达基因并进行聚类分析,荧光定量PCR验证芯片结果。结果:聚类结果显示虚寒模型组与虚热模型组基因表达轮廓具有相似性,与空白对照组有差异。虚寒模型组、虚热模型组与空白对照组相比较,存在共有差异表达基因28条,其中16条免疫应答相关基因。结论:虚寒证与虚热证大鼠均出现了免疫应答相关基因表达的显著改变,这可能是虚寒证与虚热证大鼠"精气夺则虚"的分子机制。Objective： To explain the moleeular meehanism of ＂lack of vital essence resulting in defieieney syndrome＂ in both asthenia cold syndrome rats and asthenia hot syndrome rats by gene ehip technology. Methods： The Asthenia cold and hot syndrome rats models were induced by compound preparation of Traditional Chinese Medieine. The liver gene expression in each group was detected by gene ehip. We selected the differential expression genes, analyzed the genes by duster and conducted the significant analysis on the genetic function of differential genes. A part of genes were selected to test the aeeuraey of results by RT -PCR. Results ： The cluster analysis showed the profile of genes expression of asthenia cold model group was similar to asthenia hot model group. Both asthenia cold model group and asthenia hot model group were different from control group. Among asthenia cold model group, asthenia hot model group and control group, there were 28 strips of differential expression gene, among which 16 stips were about immune response. Conclusion： Obvious changes have been both arose in asthenia cold model group and asthenia hot model group about genes immune response, which is possibly the molecular mechanism of ＂lack of vital essence resulting in deficiency syndrome＂.

关 键 词：基因芯片 虚寒证 虚热证 精气夺则虚 

分 类 号：R203[医药卫生—中医学]