长枝木霉对6种牧草种子发芽与生理效应的影响  

Effects of Trichoderma longibrachiatum on Seed Germination and Physiological Effects of Six Forage Varieties

作　　者：陈臻[1,2] 古丽君[1] 徐秉良[1] 张树武[1] 蒲崇建 

机构地区：[1]甘肃农业大学草业学院植物病理系草业生态系统教育部重点实验室甘肃省草业工程实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃省植保植检站,甘肃兰州730020

出　　处：《草地学报》2013年第3期564-570,共7页Acta Agrestia Sinica

基　　金：甘肃省农牧厅生物技术专项(GNSW-2009-04);甘肃省教育厅项目(042-03)资助

摘　　要：为了解长枝木霉(Trichoderma longibrachiatum)对牧草种子发芽及生理效应的影响,用长枝木霉T6菌株分生孢子悬浮液处理3种禾本科和3种豆科牧草的种子及幼苗,分析种子形态学指标和幼苗生理学指标的变化。结果表明:不同浓度的长枝木霉孢子悬浮液处理后,对牧草种子的发芽率、幼苗根长、芽长及干重均有明显的促进作用,与对照差异显著(P<0.05),对其生理指标也有不同程度的影响,除丙二醛含量低于对照外,叶绿素含量、根系活力、蛋白质含量、可溶性糖含量、过氧化物酶(POD)和苯丙氨酸酶(PAL)活性均显著高于对照(P<0.05)。证明了长枝木霉对牧草有明显的促生作用,并筛选出孢子悬浮液最适的促生浓度为1×106个.mL-1。To understand the effect of Trichoderma longibrachiatum on promoting growth and physiology indices of forage seed,seeds and seedlings of 3 forage grass varieties and 3 leguminous grass varieties were treated by the spore suspension of T.longibrachiatum strain T6 and seed morphological and seedling physiology indices were analyzed.Results showed that the spore suspension of T.longibrachiatum strain T6 could significantly promote forage seed germination rate,growth of the seedlings and affect physiological indicators compared with control（P〈0.05）.Except for the malondialdehyde contents,the chlorophyll,protein and soluble sugar contents as well as root,peroxidase and phenylalanine enzyme activities of treated seeds were significantly higher than that of control（P〈0.05）.These studies suggested that T.longibrachiatum strain T6 had obvious growth-promoting effects.The optimum concentration for promoting was 1×10^6 spores·mL^-1.

关 键 词：长枝木霉 促生作用 生理效应 牧草 

分 类 号：S330.2[农业科学—作物遗传育种]