小口径脱细胞基质人工血管移植后4周内膜增生的初步研究  

Hyperplasia of neointima in small-caliber decellularized matrix vascular grafts at 4 weeks after transplantation

作　　者：黄明清[1] 李翠玲 李杰 吕伟明 黎洪浩[1] 龙淼云[1] 王深明 

机构地区：[1]中山大学孙逸仙纪念医院甲状腺血管外科,广州510260 [2]山大学附属第一医院超声科,广州510080 [3]山大学附属第一医院血管甲状腺科,广州510080

出　　处：《国际医药卫生导报》2013年第15期2257-2260,共4页International Medicine and Health Guidance News

基　　金：国家863高技术研究发展计划资助项目（2006AA032441）;广东省自然科学基金项目（8151008901000191）

摘　　要：目的探讨小口径脱细胞基质（DCM）人工血管移植4周后内膜增生程度。方法将17只雄性杂种犬随机分为DCM（n=9）、膨体聚四氟乙烯（ePTFE）人工血管（n=8）2组行右颈总动脉置换术，术后1、4周彩超检测移植物吻合口及中段收缩期峰值血流速度（PSV）、舒张末期血流速度（EDV）、阻力指数（RI）及内径。术后4周活体取材，标本行HE染色及光镜检查。结果所有移植物4周内均保持通畅。DCM、ePTFE人工血管移植后4周与1周远端吻合口的RI差值（△RI）差异有统计学意义（P〈0．05），吻合口及中段其余血流动力学参数的差值差异均无统计学意义（P均〉O．05）。DCM组近端吻合口、中段及远端吻合口的内径差值（△D）则均小于ePTFE组（尸均〈0．05）。结论移植后4周小口径DCM人工血管内膜增生程度小于ePTFE人工血管。Objective To explore neointimal hyperplasia in small-caliber decellularized matrix vascular grafts at 4 weeks after transplantation. Methods 17 male dogs were randomly divided into 2 groups. All the animals underwent right common carotid artery interposition with DCM （n = 9） or ePTFE （n = 8） vascular grafts. Color Doppler Ultrasound was applied to detect peak systolic velocity （PSV）, end diastolic velocity （EDV）, resistance index （RI）, and inner diameter of anastomosis and midportion of the grafts at 1 and 4 weeks after implantation. All the grafts were harvested and evaluated by HE staining and light microscopy. Results All implanted grafts remained patent within 4 weeks. The difference of RI （A RI） at the distal anastomosis between 1 and 4 weeks differed significantly in the two groups （P〈0.05）. The difference of other hemodynamic parameters at anastomosis and midportion of the grafts did not differ significantly between the two groups （P〉0.05 for all comparisons）. The difference of inner diameter （ A D） at anastomosis and midportion was significantly smaller in DCM group than in ePTFE group （P〈0.05 for all comparisons）. Conclusions The degree of neointimal hyperplasia is smaller in small-caliber DCM vascular grafts than in ePTFE vascular grafts within 4 weeks after ttransplantation.

关 键 词：小口径 人工血管 脱细胞基质 膨体聚四氟乙烯 彩色多普勒超声 

分 类 号：R654.3[医药卫生—外科学]