Box-Behnken效应面法优化穿心莲内酯-PLGA微球处方研究  

Formula optimization of andrographolide-loaded PLGA microsphere by Box-Behnken design and response surface method

作　　者：王芳[1] 翟文婷[1] 李艳丽[1] 许卉[1] 

机构地区：[1]烟台大学药学院,山东烟台264005

出　　处：《中草药》2013年第13期1743-1747,共5页Chinese Traditional and Herbal Drugs

基　　金：烟台大学青年学术骨干培养项目资助(2012)

摘　　要：目的采用Box-Behnken实验设计方法,对穿心莲内酯微球处方进行优化。方法以聚乳酸羟基乙酸(PLGA)为药物载体,采用溶剂挥发法制备穿心莲内酯微球。分别以PLGA质量浓度、聚乙烯醇(PVA)质量浓度、油水相体积比为考察对象,以载药量和包封率为评价指标,采用Box-Behnken效应曲面法筛选穿心莲内酯微球的最佳处方。结果穿心莲内酯微球的最优处方为PLGA为0.20 g/mL,PVA为18 mg/mL,油水相体积比为1∶90(0.011),所得微球的载药量为(47.21±2.36)%,包封率为(90.15±3.48)%,与模型预测值接近。结论 Box-Behnken实验设计可用于穿心莲内酯微球的处方优化筛选。Objective To optimize the formula of andrographolide-loaded PLGA microsphere. Methods The microspheres were prepared by the solvent evaporation method using PLGA as drug carriers. The effects of the concentration of PLGA and PVA, and the oil-water phase ratio were investigated. According to the drug loading amount and encapsulation efficiency, the formula was optimized by Box-Behnken design and response surface method. Results The optimal formula was as follows： PLGA concentration of 0.20 g/mL, PVA concentration of 18 mg/mL, and oil-water phase ratio of 1：90, respectively. The drug loading amount of the microsphere was （47.21 ± 2.36）% and the encapsulation efficiency was （90.15 ±3.48）%, which were very close to the predicted values. Conclusion It is effective and feasible to apply Box-Behnken design and response surface method for the formula optimization of the andrographolide-loaded PLGA rnicrosphere.

关 键 词：穿心莲内酯 微球 处方优化 Box Behnken实验设计 效应面法 

分 类 号：R283.6[医药卫生—中药学]