银杏叶制剂中原花青素的HPLC鉴别方法研究  

HPLC determination of proanthocyanidins in Ginkgo Folium preparation

作　　者：陈燕[1] 乔洪翔 叶剑锋 何厚洪 王如伟[1,2] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江现代中药与天然药物研究院,浙江杭州310052

出　　处：《中草药》2013年第13期1774-1778,共5页Chinese Traditional and Herbal Drugs

基　　金：国家重大新药创制项目(2011ZX09203-001-18);浙江省重点实验室和中试基地计划项目(2009E10009)

摘　　要：目的建立银杏叶制剂中原花青素的定性检测方法,为原花青素的质量控制提供依据。方法建立NP-HPLC法和HPLC-DAD-MS法分析银杏叶原花青素提取物、葡萄籽提取物、银杏叶制剂及掺假银杏叶制剂中原花青素的组成。结果原花青素在正相色谱系统中,可以按聚合度的大小进行分离;葡萄籽原花青素由(表)儿茶素组成,各个聚合体在正相色谱系统中只有1个特征峰;而银杏叶原花青素由(表)没食子儿茶素和(表)儿茶素组成,有2～3个特征峰。结论该方法可用于定性分析原花青素,还可用于鉴别银杏叶制剂中是否掺假。Objective To develop a qualitative method for the quick determination of proanthocyanidins in Ginkgo Folium preparation （GFP）, and to provide the objective evidence for the quality control of proanthocyanidins. Methods NP-HPLC and HPLC-DAD-MS methods were developed to analyze the composition of proanthocyanidins in Ginkgo Folium proanthocyanidin extract, grape-seed extract, GFP, and adulterate GFR Results The proanthocyanidins were separated in normal phase mode according to their degree of polymerization. The proanthocyanidins from grape-seed extract, which consisted of （epi）catechin, had only one characteristic absorption band in HPLC chromatograms for each degree of polymerization; And proanthocyanidins of GFP, which consisted of （epi）gallocatechin and （epi）catechin, had two or three characteristic absorption bands for each degree of polymerization. Conclusion The HPLC method is applicable for the qualitative analysis ofproanthocyanidins and the identification ofanthentic and adulterated proanthocyanidins in GFP

关 键 词：银杏叶制剂 原花青素 提取物 HPLC 聚合度 

分 类 号：R283.6[医药卫生—中药学]