槟榔碱提神效果及急性毒性作用研究  

Research on effects of arecoline on refreshing and acute toxicity test

作　　者：肖冰梅[1] 肖嫩群[1] 彭买姣[1] 龚力民[1] 李春[2] 林丽美[1] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国当代医药》2013年第20期14-16,共3页China Modern Medicine

基　　金："重大新药创制"(2013ZX09201019);教育部高等学校博士学科点专项科研基金项目(20124323120004);湖南省科技厅项目(2011FJ7008);湖南省十二五药学重点学科资助项目

摘　　要：目的研究槟榔主要活性物质槟榔碱的提神及急性毒性作用,为槟榔食用安全提供参考依据。方法将昆明小鼠随机分成3组,腹腔注射各浓度的槟榔碱溶液30 s后再注射苯巴比妥钠溶液,记录小鼠抗睡眠时间,同时以无菌生理盐水及同浓度的天竺葵作对照。采用霍恩法进行槟榔碱的急性毒性试验,计算其LD_(50)并判定其毒性等级:解剖中毒小鼠,观察其病理变化。结果槟榔碱的抗睡眠时间随浓度的增大而增长,0.5 mg的槟榔碱抗1.5×10^(-4)g苯巴比妥钠溶液引起的睡眠时间可达38 min;槟榔碱的LD_(50)是430 mg/kg体重,95%可信限为295～626 mg/kg体重,属于中等毒性。中毒死亡小鼠的肝脏稍微肿大,脾脏颜色变深、体积变大,肺部颜色略变深、有出血现象、肠内有空泡样。结论槟榔碱有抗苯巴比妥钠催眠的显著作用,是一种中等毒性物。Objective To study the betel nut actives arecoline refreshing and toxic effects,to provide reference basis for eating safety. Methods KM mice were randomly divided into three groups,intraperitoneal injection of various concen- trations of arecoline solution 30 s after injection of phenobarbital sodium,the mouse anti-sleep time,while sterile saline and the same concentration of geranium control was recorded.The acute toxicity test used Horn method arecoline,to calculate its LD50 and to determine its toxicity level;Anatomy poisoned mice to observed the pathological changes. Re- suits The anti-sleep time of arecoline was increasing with growth of concentration,0.5 mg of arecoline anti-1.5×10^-4g phenobarbital sodium-induced sleep time up to 38 min;The Arecoline the LD50 was 430 mg/kg·BW,confidence limits of 295-626 mg/kg-BW the middle poison.The poisoning death of the mice a slight enlargement of the liver and spleen become darker in color,larger size,lung color slightly darker,bleeding,intestinal vacuolation. Conclusion Arecoline the significant role of the anti-phenobarbital sodium hypnosis is a moderately toxic material.

关 键 词：槟榔碱 提神 急性毒性试验 

分 类 号：R9[医药卫生—药学]