乌龙丹对大鼠胶原诱导性关节炎的影响  

The Influence of Wulongdan on Rats with Collagen-InducedArthritis

作　　者：李泽光[1] 梁华[2] 黄吉峰[1] 刘婷婷[2] 佟颖[1] 朴勇洙[1] 李奇玮[2] 李娟[2] 黄蓉[2] 

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《风湿病与关节炎》2013年第7期29-31,38,共4页Rheumatism and Arthritis

基　　金：黑龙江省中医药管理局基金资助项目(ZHY12-2063);黑龙江省自然科学基金项目(D201277)

摘　　要：目的:通过观察乌龙丹对胶原诱导性类风湿关节炎模型大鼠的影响,探讨乌龙丹抗类风湿关节炎的作用及其机制。方法:对Ⅱ型胶原诱导性大鼠建立类风湿关节炎模型,通过测定关节炎指数积分、关节肿胀率变化情况,评价乌龙丹的治疗作用,并以Elisa法检测血清及滑膜液中白细胞介素-1β、白细胞介素-6、转化生长因子-β1、白细胞介素-17F、基质金属蛋白酶-3表达情况,分析并探讨其作用机制。结果:乌龙丹各治疗组可明显降低模型大鼠关节炎指数积分,血清及滑液中白细胞介素-1β、白细胞介素-6、转化生长因子-β1、白细胞介素-17F、基质金属蛋白酶-3均有不同程度下降,其中以中剂量组下降最为显著。结论:乌龙丹对胶原诱导性类风湿关节炎模型大鼠关节炎具有良好的治疗作用,其机制可能是通过调节关节滑液及血清中白细胞介素-1β、转化生长因子-β1含量,抑制了CD4+T细胞向Th17细胞分化,从而阻断了白细胞介素-1β、白细胞介素-17F、基质金属蛋白酶-3等因子的生物学效应而达到阻止或延缓类风湿关节炎炎症和关节破坏的作用。Objective：To explore the effect and mechanism of Wulongdan on rheumatoid arthritis through observing the influence of wulongdan on rat model with collagen-induced arthritis.Methods：The rat model with rheumatoid arthritis was established by collagen type II,evaluating therapeutic effect of wulongdan by measuring arthritis index score and changes of joint swelling rate and analyzing and exploring its mechanism by measuring with elisa method the expression of interleukin-l13 in serum and synovial fluid,interleukin- 6,transforming growth factor-1β,interleukin-17F and matrix metalloproteinase-3.Results：Wulongdan in the treatment group could obviously reduce the arthritis index score of rat model,and at the same time interleukin- 1β in serum and synovial fluid,interleukin-6,transforming growth factor-1β,interleukin-17F and matrix metalloproteinase-3 decreased in various degrees,among which above factors in the middle dose group decreased signiflcantly.Conclusion：Wulongdan had good therapeutic effect on collagen-induced rheumatoid arthritis in rats,whose mechanism may be through the regulation of interleukin-1β in serum and synovial fluid,interleukin- 6,transforming growth factor-1β,interleukin-17F and matrix metalloproteinase-3 to prevent or delay the rheumatoid arthritis and joint destruction.

关 键 词：乌龙丹 白细胞介素 转化生长因子-Β1 基质金属蛋白酶-3 实验研究 大鼠 

分 类 号：R285.5[医药卫生—中药学]