心房颤动患者心耳组织肝细胞生长因子、脑钠肽与纤维化的相关研究  

Hepatocyte growth factor,B-type natriuretic peptide and atrial fibrosis in patients with atrial fibrillation

作　　者：庄端蓉[1] 陈颖敏[1] 茆臻贞[1] 李宏松[1] 徐瑾[1] 何奔[1] 

机构地区：[1]上海交通大学附属仁济医院,上海200001

出　　处：《中国心脏起搏与心电生理杂志》2013年第3期207-210,共4页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：上海市科委项目(项目编号:11140900800)

摘　　要：目的检测肝细胞生长因子(HGF)、脑钠肽(BNP)在心房颤动(简称房颤)患者心房组织中的表达,探讨心房纤维化的分子机制。方法将术中获取右心耳的26例风湿性心脏病(简称风心病)患者分为2组,其中窦性心律(简称窦律)组10例,房颤组16例,另选先天性心脏病患者6例(均为窦律)作为对照组;于术中获取右心耳组织,采用苦味酸天狼猩红染色法对心房组织胶原沉积量及分布情况进行分析,以β-肌动蛋白(β-actin)为内参照基因,采用实时荧光定量聚合酶链式反应技术测定心房组织中HGF、BNP的mRNA含量,用免疫组织化学方法评价HGF、BNP的蛋白表达情况。结果与对照组和窦律组比较,慢性房颤组心房组织胶原容积分数明显增加(P<0.01),而风心病窦律组和对照组无明显差异。与对照组和窦律组比较,房颤组HGF、BNP的mRNA和蛋白表达显著降低(P<0.05),而风心病窦律组和对照组无明显差异。结论房颤患者心房组织中HGF、BNP表达显著降低可能是导致心房纤维化的分子机制之一,与房颤的发生和维持有关。Objective To investigate the expressions of hepatocyte growth factor（HGF） and B-type natriuretid peptide （BNP） in patients with atrial fibrillation（ AF）, and discuss their possible molecular mechanisms in atrial fibrosis. Meth- ods Twenty-six patients with rheumatic heart disease（RHD） were enrolled. Ten patients had no history of AF and 16 had AF. Atrial tissue was obtained from the right atrial appendage during heart surgery. Other 6 patients with congenital heart disease who had no history of AF were taken as the control group ; Sirius red staining for quantitative analysis of collagen accumulation. The mRNA expression of HGF and BNP was measured by real-time fluorescence quota polymerase chain reaction. The protein expression of HGF and BNP was detected by immunhistochemical method. Results The volume frac- tion of collagen were significantly increased in patients with AF compared with patients with sinus rhythm and the control group（ P〈O. 01 ） , but the difference between latter two groups was of non-statistics significance. The mRNA and protein ex- pression of HGF and BNP were significantly decreased in patients with AF compared with patients with sinus rhythm and the control group（ P〈0.05 ）, but the difference between latter two groups was of non-statistics significance also. Conclusion The decreased expressions of HGF and BNP in atrium during AF may contribute to atrial fibrosis.

关 键 词：心血管病学 心房颤动 纤维化 肝细胞生长因子 脑钠肽 

分 类 号：R541.75[医药卫生—心血管疾病]