亲水性糖皮质激素衍生物对巨噬细胞呼吸爆发的影响  

Effects of Hydrophilic Glucocorticoid Derivatives on the Respiratory Burst in Macrophages

作　　者：马骞[1] 刘磊[1] 蒋春雷[1] 张璐定[1,2] 

机构地区：[1]第二军医大学航海医学教研室,上海200433 [2]第二军医大学附属长征医院,上海200003

出　　处：《现代生物医学进展》2013年第20期3823-3825,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30971083)

摘　　要：目的:观察糖皮质激素(GC)衍生物对巨噬细胞呼吸爆发的影响,并探讨GC对巨噬细胞呼吸爆发的非基因组作用机制。方法:合成亲水性和疏水性的系列GC衍生物,分离并培养腹腔巨噬细胞,应用细胞色素C还原法检测PMA刺激巨噬细胞生成超氧阴离子的能力。结果:获得了亲水性的GC衍生物氢化可的松-21-甘氨酸酯盐酸盐(HG)、氢化可的松-21-赖氨酸酯盐酸盐(HL)和疏水性的氢化可的松-21-苯丙氨酸酯盐酸盐(HP),HG和HL可显著促进PMA刺激巨噬细胞生成超氧阴离子,HP无显著作用。结论:GC快速增加PMA刺激的巨噬细胞超氧阴离子产生是通过非基因组机制实现的,这一作用与疏水性有关。Objective： To observe the effects of glucocorticoid derivatives on respiratory burst in mouse peritoneal macrophages, and identify its nongenomic phenylalanine to get hydrophilic and hydrophobic glucocorticoid derivatives. Methods： Hydrocortisone conjugated with glycine, lysine and superoxide anions were measured by cytochrome C reduction assay to observe the effects on respiratory burst of mouse peritoneal macrophages. Results： Hydrophilic glucocorticoid derivatives, hydrocortisone-conjugated glycine （HG）, hydrocortisone-conjugated lysine （HL）, and hydrophobic hydrocortisone-conjugated phenylalanine （HP） were obtained. Hydrocortisone （HC）, HG and HL could rapidly increase the superoxide anions production by macrophages, but HP did not. Conclusions： The effects of glucocorticoids on promoting the superoxide anions production by mouse peritoneal macrophages may be mediated by nongenomic mechanisms, which was related to hydro!ohobicity.

关 键 词：糖皮质激素衍生物 巨噬细胞 超氧阴离子 呼吸爆发 非基因组机制 

分 类 号：Q953[生物学—动物学] R977[医药卫生—药品]