DNA测序技术的进展和挑战  

The Developments and Challenges of DNA Sequencing Technologies

作　　者：蔡晓静[1] 朱煌[1] 孔繁琪[1] 冯婕妤[1] 赵立全[1] 牛宗镇[1] 

机构地区：[1]上海交通大学医学院附属新华医院眼科,上海200092

出　　处：《现代生物医学进展》2013年第20期3981-3984,共4页Progress in Modern Biomedicine

基　　金：上海市卫生局中医药基金(2009S023)

摘　　要：基因是人类的遗传信息的载体,其遗传和表达影响人类的繁衍及各种生命活动;个体基因组DNA序列突变往往会导致疾病的发生,获取个体的基因组DNA序列将有助于疾病的诊断。DNA测序技术潜在的医学应用前景使其近几年有了飞速的发展。本文将介绍各代DNA测序技术已经取得的进展,目前尚待克服的挑战及可能的解决办法,并着重分析最新的单分子测序技术的发展潜能及临床应用前景。Gene is the carrier of genetic information, whose replication and expression influences human＇s heredity and various life events, while the mutation of individual＇s genomic DNA is inclined to diseases. Therefore, it is useful to obtain individual＇s genomic DNA for diagnosis of diseases, and the potential medical applications of DNA sequencing technologies makes them develop more and more quickly. This paper will introduce the achievements and challenges of every generation of DNA sequencing technologies, discuss the possible solutions to the challenges, and stress on analysis of the potentials of the latest single-molecular DNA sequencing technologies and their clinical applications.

关 键 词：单分子 纳米孔 外切酶 DNA测序 

分 类 号：Q3[生物学—遗传学] Q75