RhPTH(1-34)和双磷酸盐交替联合应用对去势大鼠骨质疏松模型治疗的实验研究  

Curative Effects of Sequential Application of RhPTH1-34 and Bisphosphonates on Osteoporosis in Ovariectomized Rats

作　　者：窦志 景士忠 白峰[2] 刘剑[2] 范少地[2] 周凤金[2] 

机构地区：[1]天津武警总队医院,天津300000 [2]解放军第451医院,陕西西安710054

出　　处：《现代生物医学进展》2013年第21期4031-4034,共4页Progress in Modern Biomedicine

摘　　要：目的:研究联合应用重组人甲状旁腺素(1-34)和双磷酸盐对双侧卵巢切除(OVX)骨质疏松大鼠模型的治疗作用。方法:选用3月龄健康SD雌性大白鼠40只作为实验动物,随机分为五组:假手术组(S组)、卵巢切除组(O组)、OVX+RhPTH(1-34)组(R组)、OVX+阿仑磷酸钠组(A组)、OVX+联合用药组(RA组)。术后12周开始给药,持续给药6周。给药后6周取血行相关生化指标检测,取股骨进行骨密度测定、骨组织形态计量学分析。结果:O组的大鼠股骨骨密度值较假手术组有明显减少,血清磷、骨钙素和血清雌二醇水平减少(P<0.01),血清碱性磷酸酶水平增加(P<0.01),使用甲状旁腺素和阿仑膦酸钠治疗后,大鼠股骨骨密度值、血清磷、骨钙素和血清雌二醇水平增加,血清碱性磷酸酶水平相应下降,尤其是两种药物联合应用后效果更为明显(P<0.01);荧光双标显示,O组骨形成速率较R组及RA组低(P<0.05),S组、O组、A组骨形成速率无明显差别(P>0.05)。结论:联合应用RhPTH(1-34)和双磷酸盐可以减少去卵巢大鼠骨量的丢失,预防骨质疏松的发生。Objective： To investigate the effect of sequential application of rhPTH1-34 and bisphosphonates on osteoporosis in ovariectomized rats in order to provide evidence for its clinical application.Methods： Forty SD female rats at the age of 3 months were randomly divided into 5 groups： sham operation group（S group）,ovariectomized group（O group）,ovariectomized ＋RhPTH1-34（R group）,ovariectomized ＋Alendronate Sodium（A group）,ovariectomized ＋RhPTH1-34 and Alendronate Sodium（RA group）.The rats were given drugs after 12 weeks.Bone mineral density（BMD）,biochemical indexes,and bone histomorphometry parameters were measured with computer image analysis in tetracycline bone double labeling fluorescence were measured after treatment 6 weeks later.Results： The bone mineral density of femur and concentration of P iron,E2,BGP in group O decreased significantly,and concentration of ALP increased significantly compared with S group（P0.01）.RhPTH1-34 and Bisphosphonates improved BMD and biochemical indexes especially in RA group.The tetracycline bone double labeling fluorescence show that the mineral apposition rate in O group was lower than those in R and RA group.There were no significant differences of MAR among group S,O and A group（P0.05）.Conclusion： Sequential Application of rhPTH1-34 and bisphosphonates was effective in treating osteoporosis in ovariectomized rats,especially in improving BMD of femur.

关 键 词：骨质疏松症 甲状旁腺素 双磷酸盐 

分 类 号：Q95-3[生物学—动物学] R68[医药卫生—骨科学]