基于HPLC指纹图谱的不同产地升麻质量评价研究  

Study on quality evaluation of Cimicifugae Rhizoma from different producing areas by HPLC fingerprint

作　　者：沈保家[1,2,3] 秦昆明[1,2,3] 张星海[1] 刘启迪[1] 蔡皓[1,3] 刘晓[1,2] 蔡宝昌[1,2,3] 

机构地区：[1]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210029 [2]南京海昌中药集团有限公司,江苏南京210061 [3]国家中医药管理局中药炮制标准重点研究室,江苏南京201146

出　　处：《中国中药杂志》2013年第13期2155-2158,共4页China Journal of Chinese Materia Medica

基　　金：国家科技部火炬计划项目(2010GH020663);江苏省科技支撑计划-工业项目(BE2011012);江苏省科技成果转化项目(BA2010024);南京市科技公共服务平台建设项目(201105007);南京市科技发展计划项目(201007005)

摘　　要：建立不同产地升麻药材的指纹图谱。使用Kromasil色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%甲酸溶液,梯度洗脱,柱温35℃,流速1.0 mL.min-1,检测波长254 nm。提取12个色谱峰作为指纹图谱共有峰,采用相似度评价、聚类分析、主成分分析等方法,对所收集的21批样品进行系统比较与归类。结果确定了12个共有峰,将不同产地的样品分为3类。该法重复性好,简便可靠,可以为不同产地升麻的质量控制和评价提供依据。To establish a fingerprint for Cimicifugae Rhizoma from different producing areas. Column kromasil （4. 6 mm× 250 mm, 5 μm） was employed with acetonitrile-0. 1% formic acid solution as the mobile phase for gradient elntion. The flow rate was 1.0 mL .min-1, the detection wavelength was 254 nm. Twenty chromatographic peaks were extracted as the common peaks of fingerprint, and 21 batches of samples were compared and classified with such methods as similarity evaluation, cluster analysis and principle com- ponent analysis. The results showed 12 common peaks and three categories of samples. The method was so highly reproducible, simple and reliable that it could provide basis for quality control and evaluation of Cimicifugae Rhizoma from different producing areas.

关 键 词：升麻 指纹图谱 

分 类 号：R284[医药卫生—中药学]