甲基莲心碱对肝星状细胞Collagen-Ⅰ,TIMP-1及MMP-2的影响  

Effect of neferine on hepatic stellate cells in Collagen-Ⅰ,TIMP-1 and MMP-2

作　　者：李显梅[1] 董蕾[1] 史海涛[1] 高天娇[1] 贾淼[1] 

机构地区：[1]西安交通大学医学院第二附属医院消化内科,陕西西安710004

出　　处：《中国中药杂志》2013年第13期2206-2209,共4页China Journal of Chinese Materia Medica

摘　　要：目的:观察甲基莲心碱对肝星状细胞Collagen-Ⅰ,TIMP-1及MMP-2 mRNA表达及蛋白分泌的影响。方法:体外培养大鼠肝星状细胞株HSC-T6,随机分为5组:对照组、血小板衍生因子(PDGF)组、甲基莲心碱低、中、高剂量组(2,6,10μmol.L-1)。分别加入各组药物培养48 h后,收集细胞提取总RNA,采用RT-PCR检测Collagen-Ⅰ,TIMP-1及MMP-2 mRNA表达,并收集细胞上清液采用ELISA检测3个因子的蛋白含量。结果:与对照组相比,PDGF可明显增加肝星状细胞Collagen-I,TMIP-1及MMP2 mRNA表达及蛋白分泌。与PDGF组相比,甲基莲心碱中、高剂量组Collagen-Ⅰ及TMIP-1 mRNA表达及蛋白分泌明显减少,且随着浓度的增加,减少更明显,而甲基莲心碱低剂量组Collagen-Ⅰ及TMIP-1表达及分泌无明显变化。与PDGF组相比,甲基莲心碱3个剂量组的MMP-2 mRNA表达及蛋白分泌均无明显变化。结论:甲基莲心碱可抑制PDGF诱导的HSC的Collagen-Ⅰ和TIMP-1的mRNA表达及蛋白分泌,且随浓度的增加,抑制作用加强,但对MMP-2的表达及分泌无明显影响。Objective： To observe the effect of nefefine on Collagen-Ⅰ, TIMP-1 and MMP-2 expressions and protein secretion of hepatic stellate cells. Method： The hepatic stellate cell line HSC-T6 was cultured in vitro, and then randomly divided into 5 groups： the control group, the platelet-derived growth factor （PDGF） group and PDGF ＋ neferine （2, 6, 10 μmol . L-1） groups. All of the groups were cultured for 48 h, and their cells were collected to extract mRNA and detect Collagen-Ⅰ, TIMP-1 and MMP-2 expressions with RT-PCR. Their cell supernatants were also collected to determine the protein content of three factors with ELISA. Result： Com- pared with the control group, PDGF could remarkably increase the Collagen-Ⅰ, TIMP-1 and MMP-2 expressions and protein secretion of hepatic stellate cells. Compared with the PDGF group, PDGF ＋ nefefine （6, 10μmol. L-l） groups showed a notable decrease in the Collagen-Ⅰand mRNA expression and protein secretion along with the increase in the concentration, whereas the PDGF ＋ neferine （2 μmol .L-1） group showed no significant change in the Collagen-Ⅰ and mRNA expression and protein secretion. Compared with the PDGF group, three PDGF ＋ neferine groups showed no notable change in MMP-2 expression and protein secretion. Conclusion： Nefer- ine can inhibit the Collagen-Ⅰ, TIMP-1 and mRNA protein expression and protein secretion of PDGF-induced HSCs along with the in- crease in the concentration, but with not remarkable effect on the MMP-2 expression and secretion.

关 键 词：肝星状细胞 甲基莲心碱 Collagen-Ⅰ TIMP-1 MMP-2 

分 类 号：R285.5[医药卫生—中药学]