固相萃取富集高效液相色谱法测定卷烟烟气中的铅、镉和汞  

Determination of Lead,Cadmium and Mercury in Cigarette Smoke with Solid Phase Extraction by High Performance Liquid Chromatography

作　　者：殷晓玲[1] 王亚明[2] 彭丽娟[3] 胡秋芬[3] 

机构地区：[1]昆明理工大学理学院应用化学系 [2]昆明理工大学化学工程学院化工系 [3]云南省烟草化学重点试验室云南烟草科学研究院

出　　处：《光谱实验室》2013年第4期1670-1673,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：云南省教育厅基金项目

摘　　要：研究了用固相萃取富集,柱前衍生高效液相色谱法测定卷烟烟气中的铅、镉、汞的方法。卷烟烟气样品经剑桥滤片捕集,用四-(对甲氧基苯基)-卟啉(T4 MPP)柱前衍生卷烟烟气中的重金属元素:铅、镉、汞。采用C18固相萃取小柱萃取富集铅、镉、汞的卟啉T4MPP络合物,以Waters Xterra RPl8色谱柱为固定相,甲醇和四氢呋喃为流动相梯度洗脱,用光二级管矩阵检测器检测。铅、隔、汞的捡出限在0.02—0.03μg/L之间。方法相对标准偏差小于2.3%,加标回收率为95%—102%。该方法用于测定卷烟烟气中的重金属元素铅、镉和汞,结果可靠,令人满意。A method for the high performance liquid chromatography（HPLC） determination of lead,cadmium and mercury in cigarette smoke after preconcentraction by solid phase extraction was established.The cigarette smoke was collected using a cambridge filter,the lead,cadmium and mercury ions were pre-column derived by tera-（p-methoxy）-porphine（T4MPP）.These chelates were enriched by solid phase extraction with C 18 cartridge was achieved.The chelates were separated on a C18 stable bound by gradient elution with methanol and THF as mobile phase and detected with photodiode array detector.The detection limits of lead,cadmium and mercury are in the range of 0.02—0.03μg/L,the relative standard deviations of these trace elements are below 2.3 %,and the standard recoveries of these elements are in the range of 95%—102%.This method had been applied to the determination lead,cadmium and mercury in the cigarette smoke with good results.

关 键 词：固相萃取 高效液相色谱法 铅、镉、汞 卷烟烟气 

分 类 号：O657.72[理学—分析化学]