基于FTIR和HPLC的不同产地地枫皮槲皮苷含量比较分析  

Comparison Analysis of Quercitrin of Illicium difengpi From Different Regions Based on FTIR and HPLC

作　　者：孔德鑫 史艳财 王满莲 邹蓉 陈宗游 唐辉 

机构地区：[1]广西植物功能物质研究与利用重点实验室广西植物研究所

出　　处：《光谱实验室》2013年第4期1840-1844,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：广西科技攻关项目(10124008-1);中国科学院"西部之光"人才培养计划项目[科发人教字(2009)24];广西植物研究所基本业务费项目(桂植业11008);桂科能项目(0992028-10);广西植物功能物质研究与利用重点实验室主任基金项目(ZRJJ2012-10)

摘　　要：通过HPLC技术测定槲皮苷含量并与其药材的傅里叶红外光谱进行相关分析,研究不同产地地枫皮槲皮苷含量相关的特征峰,试图快速筛选出高槲皮苷含量的优良地枫皮种质。结果显示:不同产地地枫皮间红外吸收光谱的峰形和峰位置均比较接近,但各谱峰的峰强有一定差异;为进一步研究与槲皮苷相关的吸收峰,对1732、1613、1518、1448、1373、1281、1158、1105、1056、824、771、663cm-1等处的峰强与实测槲皮苷含量的关系进行多元对数相关回归分析,结果显示:在1613、1448、1153、663cm-1等处附近的吸收强度与实际测定不同产地地枫皮槲皮苷含量均密切相关,R2=0.9800,P=0.002;两种方法均一致显示,槲皮苷含量以靖西化洞产地最高,其次是靖西安德和来宾忻城,其中以龙州产地含量最低。To investigate the effect of different regions of I.difengpi on the quercitrin content,the quercitrin content for different regions of I.difengpi were detected and analyzed by HPLC and FTIR in this work.The results indicated that the vibration and the position of infrared absorption spectrum of I.difengpi were similar,but the intensity were had significant differences under different regions.In order to clear the relationship between spectrum absorption peak intensity and quercitrin content of I.difengpi,multiple regression analysis with logarithmic analysis using SPSS software was used to analysze the absorption peak at 1732,1613,1518,1448,1373,1281,1158,1105,1056,824,771,663cm-1,etc.R2=0.9800,P=0.002.After statistical analysis,it could be shown the significant correlation between the absorption intensity and the actual determination of quercitrin content of I.difengpi at 1613,1448,1153,663cm-1,et al.Two kinds of methods are consistently shown that the highest content of quertcitrin is the region of HD,the higher content of quertcitrin were the region of AD and XC,the lowest content was Longzhou.

关 键 词：傅里叶变换红外光谱 槲皮苷 地枫皮 

分 类 号：O657.72[理学—分析化学] O657.33[理学—化学]