一种混合物体系荧光分析消除干扰的新方法  

A New Approach for Eliminating Interference in Fluorescence Analysis of Mixture System Detection

作　　者：史训立[1,2] 张琳[1] 张英华[3] 

机构地区：[1]石家庄经济学院数理学院,石家庄市槐安东路136号050031 [2]河北师范大学化学与材料科学学院,石家庄市南二环东路20号050024 [3]青岛理工大学计算机工程学院,山东省青岛市抚顺路266520

出　　处：《光谱实验室》2013年第4期1964-1967,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：河北省自然科学基金项目(D2012403010)

摘　　要：荧光分析法在测量混合物体系时,有些组分的最大激发峰相距较近,彼此之间会因存在干扰而得不着准确的荧光光谱。以中药苦参沸水浸取液为例,介绍了混合物体系中消除干扰的一种新方法。首先通过扫描苦参水浸液的荧光光谱,发现其中一种组分B对另一种组分A有明显干扰;然后通过几何作图法在组分B激发光谱中找到具有与组分A最大激发波长处同样荧光强度(也就是与在该点等高的位置处)的波长,以该波长作为激发波长扫描得到组分B在该波长处激发而得到的发射光谱;最后以受干扰的组分A的发射光谱减去该发射光谱即得到纯组分A的发射光谱。该方法使得在无法得到组分A和B的纯品时,对二者进行单独的荧光分析成为了可能。In fluorescence analysis of mixture system,the maximal excitation peaks of some components may be close to each other,and then interferes with each other.So,exact fluorescence spectra cannot be obtained in this case.Taking the aqueous extracts of Lightyellow Sophora Root（LSR） as a working example,a new approach for eliminating the interferences in mixtures was proposed.Firstly,it was discovered that one component A has obvious interference to another component B through scanning fluorscence spectrum of the aqueous extract of LSR.Then in excitation spectrum of component B find a wavelength that has same fluorescence intensity as maximal excitation wavelength of component A,namely a point that has same height as this point.Use this wavelength as excitation wavelength scan to get the emission spectrum of component B excitated at this wavelength.At last,use the emission spectrum of component A interfered by component B minus the above emission spectrum to get the emission spectrum of pure component A.This approach makes it possible to do separately fluorescence analysis on these two components when cannot obtain the pure components A and B.

关 键 词：荧光分析 混合物体系 消除干扰 

分 类 号：O657.39[理学—分析化学] O657.32[理学—化学]