牦牛HSFY基因的克隆及其结构分析  

Yak HSFY Gene Cloning and Structure Anlysis

作　　者：曾贤彬[1] 王永[1] 刘仲娜[1] 马志杰[2] 海汀[1] 钟金城[1] 

机构地区：[1]西南民族大学动物遗传育种学国家民委--教育部重点实验室,成都610041 [2]青海大学畜牧兽医科学院,西宁810016

出　　处：《生物技术通报》2013年第7期82-88,共7页Biotechnology Bulletin

基　　金：国家科技支撑计划课题(2012BAD13B06);西南民族大学研究生创新科研项目(CX2013SZ65);中央高校基本科研业务费专项资金项目(11NZYTH03)

摘　　要：Y染色体上的热休克转录因子(HSFY)是精子发生障碍的候选基因之一。通过克隆牦牛HSFY基因,并与普通牛Y染色体上的84条HSFY序列比对。结果表明:牦牛HSFY基因由2个外显子和1个内含子组成,与普通牛基因组中的相应序列的一致性高达99.54%。牦牛、普通牛的该基因具有4处插入/缺失,且序列与其相邻序列极为相似。在1 012-1 020 bp(Ⅰ)处有AAAGAAGA/TG等9个核苷酸缺失,位于内含子内;在1 071-1 073 bp(Ⅱ)、1 249-1 251 bp(Ⅲ)处分别有AAG、CCA/C等3个核苷酸缺失,位于第二外显子内,分别导致赖氨酸和组氨酸的缺失;在1 708-1 710 bp(Ⅳ)处有CAA 3个核苷酸缺失,位于3'末端非编码区。在普通牛的84条HSFY基因序列中Ⅰ、Ⅱ、Ⅲ、Ⅳ插入/缺失分别有29、3、2、1次。未发现Ⅱ、Ⅲ同时缺失的拷贝,但Ⅱ、Ⅲ缺失均伴有Ⅰ缺失,且导致了蛋白三维结构的变化。牦牛HSFY是在睾丸中表达的多拷贝基因,经密码子偏好性分析,该基因编码的蛋白质偏好使用以A或T结尾的密码子。Heat shock transcription factor linked Y chromosome is one of the candidate genes for spermatogenesis obstacle. In this study, sequences of HSFYgenes of yak were obtained by cloning and alignmented with 84 HSFY sequences on the Y chromosome of the cow. The results show that ： The nucleotide homology of yak＇ HSFY genes which are composed with two exons and one intron with the corresponding sequences in the genome of the cow is high up to 99.54%. We found four insertion/deletion sequences. Between the 1 012-1 020 bp （ I ） , it lacks 9 nueleotides （ AAAGAAGA/TG ） which is located in the intron. While between the 1 071-1 073 bp （ II ） and 1 249-1 251 bp （ III ） lack AAG and CCA/C respectively, which are located in the second extron and that lack lysine and histidine respectively in the amino acid sequence. Between the 1 708-1 710 bp （ IV ） , there is a 3 nucleotides deletion （ CAA ）, which is located in 3＇-URT. In the 84 HSFY genes of the cow, the frequency of the insertions/deletion is 29, 3, 2, 1 for I , II , III and IV , respectively. It is not found one with both II and m deletion. But all II or III deletion are accompany with I deletion. II and III deletions change three-dimensional structure of HSFY protein. Yak HSFY which is a muhicopy gene prefers using codes that are ended with A or T.

关 键 词：牦牛 HSFY 插入 缺失 精子发生 

分 类 号：S823[农业科学—畜牧学]