苹果轮纹病菌的多基因联合鉴定  

Botryosphaeria dothidea of Multiple Genes Joint Identification

作　　者：贾广成[1,2] 周增强[2] 侯珲[2] 王丽[2] 朱建兰[1] 

机构地区：[1]甘肃农业大学,兰州730070 [2]中国农业科学院郑州果树研究所,郑州450009

出　　处：《生物技术通报》2013年第7期161-166,共6页Biotechnology Bulletin

基　　金：公益性行业(农业)科研专项项目(3-22);国家苹果产业技术体系(CAIS-28)

摘　　要：从不同的苹果种植区采集10株苹果轮纹病菌,鉴定苹果轮纹病菌和明确苹果轮纹病菌的主要致病基因的存在状况,利用PCR技术对苹果轮纹病菌的基因间隔序列(Internal transcribed spacer,ITS)、基因延伸因子1(Elongation factor 1α,EF-1α)和微管蛋白B(Beta tubulin,β-tubulin)片段进行扩增并测序。结果表明,苹果轮纹病的病原菌是Botryosphaeria dothidea,苹果轮纹病菌的基因组中有致病相关的EF-1α基因和β-tubulin基因。微管蛋白和转录因子可能参与苹果轮纹病的侵染过程,在苹果轮纹病的致病过程中可能起着重要的作用。In order to identify apple ring rot and make sure of the present situation of apple ring rot＇ s main pathogenic genes, taking the method of PCR to amplificate and sequence the ITS genes, EF-la gene aud β-tubulin gene. The results showed that the fungi of apple ring rot is Botryosphaeria dothidea. There were EF-la gene and β-tubulin gene in the apple ring rot＇s DNA. Tubulin and transcription factors may be involved in the disease process of apple ring rot, and they are important in the pathogenic process of the apple ring rot.

关 键 词：苹果轮纹病菌 PCR ITS EF-1α Β-TUBULIN 

分 类 号：S436.611[农业科学—农业昆虫与害虫防治]