卵母细胞透明带厚度和双折射值的相关性研究  

Relevance of zona pellucida thickness and birefringence of oocyte

作　　者：成俊萍[1] 何冰[1] 黄莉[1] 谭卫红[1] 薛林涛[1] 王世凯[1] 覃捷[1] 陆建柳[1] 

机构地区：[1]广西壮族自治区人民医院生殖医学与遗传中心,广西南宁530021

出　　处：《中国妇幼保健》2013年第21期3458-3460,共3页Maternal and Child Health Care of China

基　　金：广西科技厅面上基金项目〔2012GXNSFAA053130〕;广西卫生厅自筹课题〔Z2011459〕;广西卫生厅重点课题〔重2010043〕

摘　　要：目的:卵母细胞透明带厚度(ZT)和双折射值(ZB)的相关性。方法:回顾性分析了214个IVF周期中不同年龄病人、不同成熟度的卵母细胞ZB和ZT。结果:20～29岁组ZT和ZB显著高于≥40岁组(18.24±0.16μm vs.16.67±0.41μm;8.77±0.42 vs.5.25±1.72)(P<0.01),也高于30～39岁组(18.24±0.16μm vs.17.58±0.15μm)(P<0.01),(8.77±0.42 vs.7.59±0.52)(P<0.05);ZT和ZB与年龄均呈负相关,分别为rZT=-0.195和rZB=-0.200(P<0.05),ZT和ZB之间呈正相关r=0.255(P<0.01);MII期卵母细胞ZB、ZT值均显著低于GV期(7.91±0.65 vs.20.77±1.88,17.80±0.22μm vs.19.42±0.43μm)(P<0.01),也低于MI期卵母细胞(7.91±0.65 vs.10.99±1.35;17.80±0.22μm vs.19.03±0.63μm)(P<0.05)。MII期和MI期卵母细胞ZT与ZB呈正相关,相关系数分别为r=0.125和r=0.239(P<0.05),但GV期卵母细胞ZT和ZB之间无明显相关性。结论:卵母细胞ZT和ZB均随病人年龄和成熟度增加而降低,除GV期卵母细胞外,ZT和ZB存在相关关系,ZT能反映病人年龄和成熟度对卵母细胞的影响,ZT在一定程度上可以反映ZB值。Objective：To explore the relationship between zona pellucida thickness（ZT） and the stage of oocyte maturation and the patient age,and the correlation between zona pellucid a birefringence（ZB） and ZT.Methods：A retrospective study was performed on ZT and ZB of the oocytes at different maturation stage for the patients with different ages from 214 IVF-ET cycles underwent long protocol treatment.Results：The ZT and ZB in 20~29 years old group were significantly higher than those of ≥40 years old group（18.24±0.16 μm vs.16.67±0.41 μm;8.77±0.42 vs.5.25±1.72,respectively,P＆lt;0.01）,also higher than those of 30~39 years old group（18.24±0.16 μm vs.17.58±0.15 μm,P＆lt;0.01,8.77±0.42 vs.7.59±0.52,P＆lt;0.05,respectively）.The negative correlation between age and ZT or ZB were indicated in present study with rZT=-0.195 and rZB=-0.200（P＆lt;0.05）,while the relationship between ZT and ZB was positive correlation with r=0.255（P＆lt;0.01）.The ZB and ZT of MII oocytes were significantly lower than those of GV oocytes（7.91±0.65 vs.20.77±1.88,17.80±0.22 μm vs.19.42±0.43 μm）（P＆lt;0.01）,also lower than those of MI oocytes（7.91±0.65 vs.10.99±1.35;17.80±0.22 μm vs.19.03±0.63 μm）（P＆lt;0.05）.For the correlation between ZB and ZT,positive correlation was demonstrated in MII and MI oocytes with r=0.125 and =0.239（P＆lt;0.05）.However,the correlation between ZT and ZB was not evident in GV oocytes.Conclusion：ZT could reflect the change of oocyte maturation and patient age and the ZT is decreased with oocyte maturation and patient age.ZT also could reflect the ZB of oocyte to a certain extent for the positive correlation between ZT and ZB in MII and MI oocytes.

关 键 词：卵母细胞 体外受精 透明带厚度 透明带双折射值 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]