异精雌核发育草鱼F_1与普通草鱼的SCAR标记鉴别初报  

Preliminary Report on SCAR Maker for Distinguishing Allogynogenesis Grass Carp F_1 and Common Grass Carp

作　　者：刘敏[1] 肖调义[1] 孙念[1] 刘巧林[1] 苏建明[1] 许宝红[1] 

机构地区：[1]湖南农业大学动物科学技术学院,长沙410128

出　　处：《水生态学杂志》2013年第3期94-100,共7页Journal of Hydroecology

基　　金：国家自然科学基金面上项目(31272652);国家"863"计划(2011AA100404)

摘　　要：以赤眼鳟生理失活精子诱导的雌核发育草鱼F1群体(CC)和与之母本同源的普通草鱼群体(PC)为材料,采用RAPD和SCAR分子标记相结合的技术,在100条RAPD随机引物扩增的16条有效引物中扩增筛选出7条群体差异条带,其中1条为异精雌核发育草鱼群体中特有,其余均表现为异精雌核发育草鱼群体缺失;对7条RAPD特异条带的回收中仅得到S32和S336的特异条带。经回收、克隆、测序后根据序列信息设计了4对SCAR引物,其中S336的2对SCAR引物在两群体间扩增出的条带一致,特异性消失;S32的2对SCAR引物能扩增出两群体间的特异条带;对S32-Scar1F/S32-Scar1R扩增出的SCAR标记S321643进行大样本检测结果表明:该标记在异精雌核发育草鱼群体中的出现频率为0(0/55),在普通草鱼群体中出现频率为76.7%(46/60)。The genomic DNA from common grass carp （PC） and allogynogenesis grass carp F1 （CC） induced by inactivate sperms of squaliobarbus curriculus were isolated. Using RAPD and SCAR technology, 16 primers with high repeatability and stability were selected from the 100 RAPD random primers, and 7 specific DNA fragments had been discovered. One of the fragments was specific in allogynogenesis grass carp F1, while the others only appeared in common grass carp variety. After purification, cloning and sequencing, only two specific fragments amplified by S32 and S336 had been obtained. Based on their sequence information, SCAR primers were designed. However, the PCR products amplified by S336-Scar1F/S336-Scar1R were the same between two populations, and so were SCAR primers S336-Scar2F/S336-Scar2R. Fortunately, there was difference between common grass carp variety and allogynogenesis grass carp F1 varity when amplified by S32-Scar1F/S32-Scar1R and S32-Scar2F/S32-Scar2R. 115 individuals originated from 2 populations, including 55 CC and 60 PC, were used to verify the reliability of the SCAR maker S321643 （amplified by S32-Scar1F/S32-Scar1R）. The results showed S321643 appeared in 46 PC individuals （76.7%）, while disappeared in all 55 CC individuals （0%）.

关 键 词：异精雌核发育草鱼 RAPD SCAR标记 群体鉴别 

分 类 号：S917.4[农业科学—水产科学]