铜胁迫下硫对玉米叶片叶绿素及MDA含量的影响  

Effect of Sulfur on the Content of Chlorophyll and MDA of Maize under Copper Stress

作　　者：孔大为[1,2] 贾蓉[2] 周莉娜[2] 

机构地区：[1]天津市塘沽环保监测站,天津塘沽300450 [2]西北农林科技大学资源环境学院,陕西杨凌712100

出　　处：《西北农业学报》2013年第5期25-29,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：黄土高原土壤侵蚀与旱地农业国家重点实验室基金(10501-178)

摘　　要：以农大108号玉米为供试材料,通过室内盆栽方法,测定Cu(+Cu)和S(+S)处理及二者共同处理(+Cu+S)下玉米苗期和拔节期生物量、叶片SPAD值及丙二醛(MDA)含量变化。结果表明,不同处理在玉米苗期和拔节期的生物量分别表现为CK>+S>+Cu+S>+Cu及+S>CK>+Cu+S>+Cu,说明铜污染对玉米生长有明显的抑制作用,而增加硫营养可减轻铜对植株的毒害。玉米苗期不同处理间叶绿素含量差异显著。在拔节期,+Cu处理对SPAD值的影响显著增加,但补充硫营养后可明显减小铜对叶绿素的抑制作用。苗期铜对叶片MDA含量的影响显著,可导致MDA含量增大,说明铜胁迫可引起膜质过氧化程度加剧,其产物MDA的积累增加;+S与CK处理间变化不明显,但+Cu+S较+Cu处理的MDA的积累明显减少,说明硫营养体现出一定的抵御铜胁迫的作用。Taking maize cultivar Nongda 108 as the plant material, an indoor pot experiment was conducted to investigate the changes of biomass, SPAD and MDA during the maize seeding and jointing stage with sulfate under copper stress. The results showed that the biomass was in the order of CK〉 ＋S〉＋Cu＋S〉＋Cu in the seeding stage and ＋S〉CK）＋Cu＋S〉＋Cu in the jointing stage, illustrating the significant inhibition of copper pollution on maize growth and the protective effect of sulfur nutrition on copper pollution. The difference of SPAD among the tested treatments during the seed- ling stage was significant. In the jointing stage, the addition of copper resulted in a significant increase of SPAD and the supplement of sulfur nutrition lead to an inhibition effect of copper on SPAD. During the seeding stage MDA increased with the addition of copper, which suggested copper stress could aggravate the lipid peroxidation and increase the accumulation of MDA. The maize had a comparable MDA content in the ＂＋S＂ and CK treatment, whereas the accumulation of MDA in the ＂＋Cu＋S＂ treatment was significantly decreased compared with the ＂＋Cu＂ treatment, further indicating the role of sulfur nutrition in copper stress defense.

关 键 词：硫营养 铜胁迫 SPAD MDA 玉米 

分 类 号：S513[农业科学—作物学]