兰州市部分地区鸡马立克病流行病学调查  

Epidemiological Investigation on Marek’s Disease in Some Regions of Lanzhou

作　　者：艾文娜[1] 吴润[1,2] 刁小龙[1] 李叙涟[1] 何轶群[1] 管宏伟[1] 罗鹏[1] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,兰州730046

出　　处：《西北农业学报》2013年第5期186-190,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31160510);甘肃省自然科学研究基金(1107RJZA198)

摘　　要：旨在了解兰州市及其周边地区鸡马立克病的流行情况及流行毒株meq基因的分子特征,对该病的进一步预防及控制提供参考。随机采集集约化养殖场950份羽髓和950份血液样品,进行琼脂免疫扩散试验、PCR检测及meq基因的检测和序列比对。结果表明,马立克的平均抗原阳性率为1.47%,PCR检测阳性率为2.52%,meq基因检测阳性率为1.37%,此位点4株突变株与MDV各参考毒株同源性为98.8%～100.0%。可见,所得突变株都具有强毒株的特征,突变与毒力强弱存在一定相关性。In order to determine the prevalence of Marek＇s disease （MD） in Lanzhou and neighboring area and characterize the meq gene of virulence strains and to provide some references for preventing and controlling MD, 950 samples of feather pulps and whole blood were collected to conduct agar gel immunodiffusion test（AGID）, PCR and gene detection and sequence alignment of meq gene. The result indicated that the positive rate of Marek＇s disease antigen, PCR and meq genetic testing was 1.47%, 2.52% and 1.37$,respectively, and the homology identities between reference strains and the four mutant strains were 98.8%-100.0%. This implied the mutant stains had some features of virulent strain and there was some relevance between mutation and virulence.

关 键 词：马立克病 流行病学 琼脂免疫扩散 PCR MEQ基因 

分 类 号：S858.31[农业科学—临床兽医学]