香蕉幼苗对PEG模拟水分胁迫下氮钾营养的生理响应  

Physiological Response of Banana Seedlings to PEG Simulated Drought Stress Under Different Nitrogen and Potassium Nutrition

作　　者：熊代群[1,2] 管利民 张盈盈[2,3] 常春荣[3] 黄承和[3] 黄雄军[3] 吴志祥[2,3] 

机构地区：[1]海南省农垦科学院农产品加工技术研发中心,海口570206 [2]农业部儋州热带作物科学观测实验站,海南儋州571737 [3]海南大学,海口570228

出　　处：《中国农学通报》2013年第19期134-141,共8页Chinese Agricultural Science Bulletin

基　　金：农业部儋州热带作物科学观测实验站运行费项目;华南热带农业大学校基金项目"水肥耦合对香蕉营养与生殖生长及品质的影响"(Rnd0606);中国热带农业科学院橡胶研究所;海南大学农学院提供试验平台与资助

摘　　要：为了探索香蕉作物抗旱生理对适宜的水、氮、钾组合互作效应的响应特征,水培试验中利用PEG模拟水分胁迫,研究香蕉幼苗在不同浓度的氮钾肥交互效应下,叶片相对含水量、根系活力、脯氨酸酶含量、过氧化物酶活性的变化,进行多重比较分析最佳抗旱效果的氮钾浓度组合。结果表明,水分胁迫对香蕉幼苗叶片POD酶活性和Pro含量的影响来说,均表现为PEG30%>PEG20%>PEG10%>CK(0%)。对试验因子间的交互作用进行分析表明:(1)叶片相对含水量的大小顺序:PEG5%与N50mg/L>PEG15%与N150mg/L≈PEG10%与N100mg/L;K150mg/L>K200mg/L>K100mg/L,且相互之间差异达到极显著。(2)香蕉幼苗根系活力大小顺序为:处理6(PEG10%、N150mg/L、K150mg/L)>处理5(PEG10%、N100mg/L、K100mg/L)>处理7(PEG15%、N50mg/L、K150mg/L),此3个处理的根系活力显著高于其他处理的根系活力,但三者之间差异不显著。(3)香蕉幼苗脯氨酸含量表现为处理9(PEG15%、N150mg/L、K100mg/L)比处理1(PEG5%、N50mg/L、K100mg/L)的脯氨酸含量高出2.16倍。处理9极显著高于其他处理的脯氨酸含量。(4)香蕉幼苗过氧化物酶活性表现为:处理9(PEG15%、N150mg/L、K100mg/L)比处理1(PEG5%、N50mg/L、K100mg/L)的过氧化物酶活性高出1.46倍。处理9极显著高于其他处理的过氧化物酶活性。适当的氮、钾浓度可提高香蕉幼苗叶片相对含水量、根系活力、脯氨酸含量和过氧化物酶活性,从而减轻水分胁迫的不利影响。本试验中处理6(PEG10%、N150mg/L、K150mg/L)为最佳的水、氮、钾的处理。In order to explore the response characteristics of banana drought resistance to the suitable water, nitrogen, potassium interaction, the hydroponic experiment had been done. In this experiment, the leaf relative water content, root vitality, proline enzyme content, peroxidase activity changes of banana seedlings werestudied and multiple comparisons analysis of the best drought effects combination of nitrogen and potassium concentration were done too, under different concentrations of nitrogen and potassium interaction by using PEG simulated drought stress. The results showed that： the impact of water stress on POD activity and Pro content of banana seedling leaves showed： PEG 30% PEG 20% PEG 10% CK （0% ）. Analysis showed that： the interaction between the test factor： （1） water content of leaf size： PEG 5% N 50 mg/LPEG 15% N 150 mg/L≈PEG 10% N 100 mg/L; K 150 mg/LK 200 mg/LK 100 mg/L, and differences were significant between them. （2） Root activity of banana seedlings sequence： treatment 6 （PEG 10% , N 150 mg/L, K 100 mg/L） treatment 5 （PEG 10% , N 100 mg/L, K 100 mg/L） treatment 7 （PEG 15% , N 50 mg/L, K 150 mg/L）. The root activities of the three treatments were significantly higher than the others, but there was no significant difference between them. （3） The proline content of treatment 9 （PEG 15% , N 150 mg/L, K 100 mg/L） was 2.16 times of treatment 1 （PEG 5% , N 50 mg/L, K 100 mg/L） of banana seedlings. Proline content of treatment 9 was significantly higher than those of other treatments. （4） The peroxidase activity performance of treatment 9 （PEG 15%, N 150 mg/L, K 100 mg/L） than treatment 1 （PEG 5%, N 50 mg/L, K 100 mg/L） was 1.46 times of treatment 1. Peroxidase activity of treatment 9 was significantly higher than that of other treatments. Appropriate concentrations of nitrogen and potassium could improve the leaf relative water content, root activity, proline content and peroxidase activity of banana seedling to reduce the adverse effect of

关 键 词：香蕉幼苗 氮钾营养 PEG 水分胁迫 生理指标 

分 类 号：S668.1[农业科学—果树学]