Ghrelin慢性给药对小鼠新物体和新位置识别的影响  

EFFECT OF CHRONIC GHRELIN ADMINISTRATION ON RECOGNITION OF NEW OBJECT AND LOCATION IN MICE

作　　者：赵志红[1,2] 刘天蔚[3] 马泽刚[1] 赵仁亮[2] 周宇[1] 

机构地区：[1]青岛大学医学院生理学教研室,山东青岛266021 [2]青岛大学医学院附属医院神经内科,山东青岛266021 [3]青岛大学医学院脑血管病研究所,山东青岛266021

出　　处：《青岛大学医学院学报》2013年第3期199-201,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金资助项目(3117107)

摘　　要：目的探讨慢性腹腔注射Ghrelin对C57Bl6J小黑鼠新位置和新物体识别的影响。方法将小鼠随机分为Ghrelin组和对照组,Ghrelin组小鼠腹腔注射Ghrelin 80μg/kg,对照组腹腔注射等体积的生理盐水,每天一次,连续注射8d。采用高架十字迷宫、旷场实验、新位置和新物体识别实验进行行为学测试。结果连续腹腔注射Ghrelin 8d后,小鼠不能识别物体位置的改变,但可以清楚地区分出新旧物体。而对照组小鼠可明确地分辨出新旧物体及物体位置的改变。Ghrelin组与对照组小鼠在测试时对物体总的探究时间相同。腹腔慢性注射Gh-relin不改变小鼠的自发活动和焦虑状态。结论慢性腹腔注射Ghrelin选择性抑制小鼠对物体新位置的识别和记忆能力,表明慢性腹腔注射Ghrelin对海马依赖的空间学习及记忆可能有抑制作用。Objective To explore the effect of chronic intraperitoneal injection of Ghrelin on recognition of new object and location in C57B16J mice. Methods Experimental mice were randomized to Ghrelin group and control group. Ghrelin （80 μg/kg） was given intraperitoneally, for those in the control group, same volume normal saline was offered-once a day, for 8 days. Behavior test was conducted by using elevated plus-maze, open field, new obiect identification and identification of new posi- tion. Results After 8 day intraperitoneal Ghrelin, the mice could not identify the changes of position of the objects, but could clearly distinguish between old and new objects. Those in the control group could clearly distinguish between old and new objects as well as changes of the position. The time spent in probing the objects was same in mice between the two groups. Chronic injection of Ghrelin did not alter locomotor activity and anxiety state in the experimental mice. Conclusion Chronic intraperitoneal injec tion of Ghrelin selectively inhibits mouse＇s recognition and memory in new ohiects, which indicates that the injection of Ghrelin may play an depressant action on hippocampus-dependent spatial learning and memory.

关 键 词：GHRELIN 小鼠 神经行为学表现 

分 类 号：R965[医药卫生—药理学]