急性肠梗阻大鼠血浆降钙素原和C-反应蛋白变化  

CHANGES OF PLASMA PROCALCITONIN AND C-REACTIVE PROTEIN IN RATS WITH ACUTE BOWEL OBSTRUCTION

作　　者：赵言顺[1] 冯志鹏[1] 王骥平[2] 王培戈[1] 

机构地区：[1]青岛大学医学院附属医院急诊普外科,山东青岛266003 [2]青岛市市立医院东院急诊外科

出　　处：《青岛大学医学院学报》2013年第4期362-364,367,共4页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨大鼠急性肠梗阻不同时间段内的血浆降钙素原(PCT)及C-反应蛋白(CRP)水平变化。方法 Wistar大鼠72只,随机分为正常对照组(未作任何手术处理,n=8)、假手术组(仅行开腹手术,n=32)及肠梗阻组(回盲部上5cm处结扎肠管,n=32),各组分别于模型制备手术后8、24、48、72h取下腔静脉血,采用酶联免疫吸附(ELISA)法测定血浆PCT、CRP浓度。结果肠梗阻组血浆PCT、CRP均于术后8h开始持续升高,至72h达到最高峰,组内各时间点比较差异均有显著性(F=411.303、318.77,P<0.01)。与假手术组比较,肠梗阻组血浆PCT、CRP水平在48、72h明显增高,差异有显著性(t=30.564～95.741,P<0.05)。肠梗阻组血浆PCT与CRP水平呈正相关(r=0.912,P<0.01)。结论 PCT、CRP可以作为评价肠道屏障损伤程度的指标。Objective To explore plasma procalcitonin （PCT） and C reactive protein （CRP） levels in rats with acute bowel obstruction at different time points. Methods Wiatar rats were randomly divided into normal control group （without any surgical treatment, n=8）, sham operation group （only laparotomy, n= 32）, and acute bowel obstruction group （the intestinal was ligated 5 em above ileocecal junction, n=32）. Blood sampies were taken from inferior vena cava at 8,24,48 and 72 hours after completion of the models for detection of plasma CRP and PCT by using EI.1SA. Results The PCT and CRP levels started to rise at eight hours after surgery and reached their peak at 72 hours, the difference between each time point in the group was signifi- cant （F=411.303,318.77;P〈0.O1）. Compared with the sham operation group, the PCT and CRP levels in bowel obstruction group at 48 h and 72 h time point were obviously elevated, the differences between the two groups were significant （t= 30.564 95.741 ,P〈0.05）. The levels of PCT was positively correlated with CRP in the obstruction group （r=0.912 ,P（0.01）. Conclusion The plasma PCT and CRP can be used as indicators to assess the degree of damage of intestinal barrier.

关 键 词：肠梗阻 降钙素原 C-反应蛋白 大鼠 Wistar 

分 类 号：R656.7[医药卫生—外科学]