人脂肪干细胞对大鼠带真皮下血管网皮片移植的影响  

EFFECTS OF HUMAN ADIPOSE-DERIVED STEM CELLS ON SURVIVAL OF SUBCUTANEOUS VASCULAR NET SKIN GRAFT IN RATS

作　　者：马天华[1] 陈振雨[1] 陈璐[1] 冷向峰[1] 姚如永[2] 

机构地区：[1]青岛大学医学院附属医院医学美容中心,山东青岛266003 [2]青岛大学医学院中心实验室,山东青岛266003

出　　处：《齐鲁医学杂志》2013年第3期241-243,246,共4页Medical Journal of Qilu

摘　　要：目的探讨人脂肪干细胞(ADSCs)对大鼠带真皮下血管网皮片移植成活的影响及其机制。方法体外培养增殖ADSCs,流式细胞术鉴定,应用EdU标记ADSCs。于大鼠背部设计术区,实验组(n=10)局部注射EdU标记ADSCs PBS悬液,对照组(n=10)注射PBS。术后7d处死大鼠,比较两组移植皮片的成活率;取移植皮片组织行苏木精-伊红染色,光镜下观察微血管数目;免疫组织化学染色观察组织中CD31及血管内皮生长因子(VEGF)的表达;EdU标记检测ADSCs在移植皮片内的分布及分化。结果实验组大鼠移植皮片成活率、组织单位面积内微血管数目、VEGF表达均高于对照组,差异有显著性(t=3.870～14.729,P<0.05)。实验组EdU标记ADSCs阳性细胞分化为部分小血管内皮细胞,对照组检测不到EdU阳性ADSCs细胞分布。结论局部移植的ADSCs可以在组织内分化成血管内皮细胞,并促进VEGF等生长因子的分泌,从而促进大鼠带真皮下血管网皮片移植成活。Objective To study the influence of human adipose derived stem cells （ADSCs） on survival of subcutaneous vascular net skin graft in rats and its mechanism. Methods ADSCs were cultured in vitro, assayed by flow cytometry and la beled with EdU （5-Ethynyl-2＇-deoxyuridine）. Skin graft areas were designed on the middle back of the rats. The rats in experimen tal group （n = 10） were injected with labeled ADSCs PBS, and those in the control group （n = 10） only PBS. The rats were killed seven days after operation. Survival rate of the skin grafts was compared between the two groups. The skin grafts were stained with HE for investigating microvessel count under a light microscope; the expressions of CD31 and vascular endothelial growth factors （VEGF） were detected by using immunohistoehemical staining, and the distribution and differentiation of ADSCs in transplanted flaps were labeled with EdU and detected. Results The survival rate of transplanted flaps, the number of microvessel in a unit area, and the expression of VEGF in the experimental group were higher than the control, being significant of the differences be- t ween t he two groups （t = 3.870 -- 14.729, P〈 0.05 ）. EdU positive cells differentiated into part of vascular endothelial cells in ex- perimental group while no EdU-positive cells were detected in the control. Conclusion Locally transplanted ADSCs can be dif- ferentiated into vascular endothelial ceils, promote secretion of VEGF, and motivate the survival of the grafts.

关 键 词：脂肪组织 干细胞 皮肤移植 组织工程 

分 类 号：R622.1[医药卫生—整形外科]