一种基于多壁碳纳米管的高灵敏捕获水样中细菌的纳米富集钓竿装置  

A new highly sensitive multi-walled carbon nanotubes based nano-fishing enriching rod for bacteria in water sample

作　　者：列少青[1,2,3] 李頔[1,2] 曾巧玲[3] 黄承志[1,2,3] 

机构地区：[1]发光与实时分析教育部重点实验室 [2]西南大学化学化工学院,重庆400715 [3]西南大学药学院,重庆400715

出　　处：《中国科学：化学》2013年第7期889-894,共6页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(21035005);重庆市百名杰出科技领军人才培养计划资助

摘　　要：碳纳米管因其独特的性质在生物检测方面具有广泛应用.本文基于DNA与多壁碳纳米管的相互作用而制成了作为一种新型纳米钓竿并用于水样中细菌的富集和检测.该钓竿的制备首先使用戊二醛为连接剂将经硅烷化处理的方形石英毛细管与氨基修饰的DNA相连,再依据单链DNA能缠绕多壁碳纳米管的性质将多壁碳纳米管固载在石英毛细管上制成一个纳米富集的钓竿装置.由于多壁碳纳米管与细菌细胞膜有较强的天然亲和力,因而能主动捕获细菌.实验证明,以大肠杆菌为目标检测菌,"纳米鱼竿"最短检测时间为15min,检测限为6.25×10CFU/mL,可以对饮用水等进行实时快速的细菌总数限量检测.Owing to the distinct properties,carbon nanotubes have been widely applied in biologic field.In this contribution,multi-walled carbon nanotubes were used as a nano-fishing enriching rod in targeting detection of bacteria in water sample.Single-stranded DNA （ssDNA） modified by amino group was linked to the silanized capillary through glutaral and then MWCNTs were immobilized through π-π interaction between DNA bases and MWCNTs,producing a nano-fishing rod device.Due to the high affinity between MWCNTs and cytomembrane,the device exhibited ability to actively hunt and capture the bacteria in aqueous solutions.It was found that using E.coli as targeted bacteria,the optimal detection time of nano-fishing rod device is 15 min and the detection limit is 6.25×10 CFU/mL.The novel device can find applications in real-time detection of total bacterial number in drinking water .

关 键 词：多壁碳纳米管 纳米钓竿 细菌 DNA 

分 类 号：TB383.1[一般工业技术—材料科学与工程]