He-Ne激光和增强UV-B辐射对小麦幼苗核蛋白的影响  

Effects of He-Ne Laser and Enhanced Ultravlolet-B Radiation on Nucleoprotein of Wheat Seedlings

作　　者：魏小丽[1] 马爱珍[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《激光生物学报》2013年第2期125-130,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金资助项目(30671061);山西省自然科学基金资助项目(20081101)

摘　　要：采用低剂量(5 mW.mm-2)He-Ne激光辐照经增强UV-B辐射处理(10.08 KJ.m-2.d-1)后的‘ML7113’小麦幼苗,待小麦幼苗长7 d后,对各处理组小麦幼苗的核蛋白进行提取,通过紫外吸收法测定各个处理组幼苗核蛋白的含量。采用SDS聚丙烯酰胺凝胶电泳法(SDS-PAGE)进行电泳。结果显示:小麦幼苗经UV-B辐射后核蛋白含量明显增高;单独激光处理后,小麦幼苗核蛋白含量低于对照组;经He-Ne激光和增强UV-B辐射复合处理后,小麦幼苗核蛋白含量低于UV-B辐射处理组,而高于对照组,推测一定剂量的He-Ne激光在一定程度上抑制了增强UV-B辐射的促进作用。The 7 d seedlings of wheat （‘ ML7113＇）were exposed to He-Ne laser irradiation（L）with 5 mW · mm^-2 power density after enhanced UV-B radiation （ 10.08 KJ.m^-2.d^-1）. Nucleoprotein were extracted in each treatment group. The contents of nucleoprotein of processed seedlings were measured by speetrophotometery and assayed by SDS-PAGE. The results shows that the level of nucleoprotein in the seedlings treated with UV-B was higher than that in CK group. In the seedlings irradiated by He-Ne laser is was lower than that in CK group. In the seedlings irradiated by both He-Ne laser and UV-B, the contents of nucleoprotein were lower than that in the seedlings exposed to UV-B handling but higher than those in CK group, it is speculated that a certain dose of the He-Ne laser may inhibit the role of enhanced UV-B radiation.

关 键 词：小麦 HE-NE激光 UV-B辐射 核蛋白 

分 类 号：Q512[生物学—生物化学]