冷应激小鼠十二指肠5-羟色胺免疫活性细胞的免疫组织化学研究(英文)  

Research on 5-HT Immunoreactive Cells in the Duodenum of Cold Stress Mouse by the Method of Immunohistochemistry

作　　者：陈晶[1] 肖莉杰[1] 曹宁[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319

出　　处：《激光生物学报》2013年第2期142-146,共5页Acta Laser Biology Sinica

基　　金：黑龙江省青年科学基金项目(QC2009C115)

摘　　要：采用ABC(avidin-biotin-peroxidase complex)免疫组织化学方法,观察冷应激小鼠十二指肠内5-羟色胺免疫反应阳性(5-HTIR)细胞的形态、分布位置及数量。结果显示:冷应激小鼠十二指肠内5-HTIR细胞主要分布于肠上皮细胞间,细胞形态多样,除圆形和锥形外,还具有条形和梭形。冷应激小鼠十二指肠内5-HTIR细胞数量(3.83±1.29)与对照组小鼠十二指肠内5-HTIR细胞数量(2.37±1.10)有显著差异(P=0.000)。分析表明,冷应激对小鼠十二指肠5-HTIR细胞的分布具有一定影响。The ABC immunohistochemistry method was used to investigate the shape, distribution and quantity of 5- Hydroxtryptamine immunoreactive（5-HTIR） cells in the duodenum of cold stress mouse. The results showed that 5-HTIR cells were distributed between epithelia of duodenum. The 5-HTIR cells had different shapes including round, cone, strip and fusiform. There was a distinct difference （ P = 0. 000） between the quantity of 5-HTIR cells （ 3.83 ± 1.29 ） in the duodenum of cold stress mouse and the quantity of 5-HTIR cells（2.37 ± 1.10）in the duodenum of mouse of control group. It is demonstrated that cold stress has a certain impact on the distribution of 5-HTIR cells in the duodenum of mouse.

关 键 词：冷应激 小鼠 十二指肠 5-羟色胺 免疫组织化学 

分 类 号：R446.6[医药卫生—诊断学]