拉萨藏族献血人群HPA-1～6、15基因多态性  

The polymorphism of HPA-1～6,15 in Tibetan blood donors from Lhasa

作　　者：李梦丹[1] 王珏[1] 宋宁[1] 辛友盼 李晓娟[2] 扎西顿珠[2] 刘忠[1] 陈强[1] 

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052 [2]西藏自治区血液中心

出　　处：《中国输血杂志》2013年第6期514-517,共4页Chinese Journal of Blood Transfusion

基　　金：卫生部卫生公益性行业科研专项(200902008)

摘　　要：目的了解血小板特异性抗原(HPA)-1～6、15基因多态性在拉萨藏族献血人群中的分布特征。方法采用聚合酶链式反应-序列特异性引物(PCR-SSP)技术对405名藏族无关献血者作HPA-1～6、15基因分型。结果405名无关献血者HPA各等位基因的频率分别为:HPA-1a:0.9383,HPA-1b:0.061 7;HPA-2a:0.971 6,HPA-2b:0.028 4;HPA-3a:0.617 3,HPA-3b:0.382 7;HPA-4a:0.992 6,HPA-4b:0.007 4;HPA-5a:0.949 4,HPA-5b:0.050 6;HPA-6a:0.991 4,HPA-6b:0.008 6;HPA-15a:0.603 7,HPA-15b:0.396 3。人群资料比对结果显示,与南方汉族人群(n=1 554)相比,拉萨藏族献血人群HPA-1、HPA-2、HPA-3、HPA-4、HPA-5、HPA-15系统基因频率具有明显差异;与新疆柯尔克孜族人群(n=105)相比,仅HPA-2系统基因频率具有明显差异。拉萨藏族献血人群HPA-1b等位基因频率明显低于高加索人群(n=119),而与新疆柯尔克孜族人群(n=105)相近,但明显高于亚洲其他黄种人群。结论拉萨藏族献血人群的HPA各位点基因频率显示出明显的民族和地域性特征。Objective To investigate the allele distribution of HPA-1 - 6, 15 in Titetan blood donors from Lha- sa. Methods The genotyping of HPA-1 - 6,15 were performed by the PCR with sequence-specific primers （PCR-SSP）. A total of 405 healthy volunteer unrelated Tibetan blood donors were included. Results The allele frequencies of HPAs for 405 Tibetan individuals were HPA-la： 0. 9383,HPA-lb： 0.061 7 ;HPA-2a： 0. 97i 6,HP.A-2b： 0. 028 4; HPA-3a： 0. 617 3, HPA-3b ： 0. 382 7 ;HPA-4a： 0. 992 6 ,HPA-4.b ： 0. 007 4.; HPA-Sa： 0. 949 4 ,HPA-5 b ： 0. 050 6 ; HPA-6a ： 0. 991 4, HPA-6b ： 0. 008 6 ;HPA-15a： 0. 603.7 ,HPA-15b ： 0. 396 3 ,respectively. The comparison with data of other ethnic groups showed that the allele frequencies of HPA-1 ,HPA-2,HPA-3 ,HPA.4,HPA-5 and HPA-15 were all significantly different between Tibetan and South Chinese Han（ n = 1 554）. While compared with Xinjiang Khalkhas （ n = 105）, such a significant difference was only found in HPA-2 system. The HPA-lb allele frequency in the Tibetan was significantly lower than that found in Caucasian from Germany （ n = 119 ）, but signiiicantly higher than the other compared Mongolian race; except for Xinjiang Khal- khas. Conclusion Our study demonstrates that the distribution of HPA-1 - 6,15 in Tibetan exhibit unique characteristics.

关 键 词：人类血小板抗原 多态性 藏族 PCR-SSP 

分 类 号：R457.11[医药卫生—治疗学]