基于mtCOII基因对山东省越冬代亚洲玉米螟不同种群的遗传结构分析  

Genetic structure of the overwintering Asian corn borer,Ostrinia furnacalis(Guenée) collections in Shandong of China based on mtCOII gene sequences

作　　者：李丽莉[1] 于毅[1] 国栋[2] 陶云荔[2] 褚栋[2] 

机构地区：[1]山东省农业科学院植保所,山东省植物病毒学重点实验室,济南250100 [2]青岛农业大学农学与植物保护学院,山东省植物病虫害综合防控重点实验室,青岛266109

出　　处：《生态学报》2013年第14期4377-4386,共10页Acta Ecologica Sinica

基　　金：现代农业产业技术体系专项资金CARS-02;"泰山学者"建设工程专项

摘　　要：亚洲玉米螟Ostrinia furnacalis(Guenée)是我国重要农业害虫。为了全面揭示亚洲玉米螟遗传分化以及山东省亚洲玉米螟不同种群的遗传结构,对山东省越冬代亚洲玉米螟不同种群mtCOII基因序列与来自GenBank的相关序列进行了遗传结构分析,并建立了鉴别不同遗传支系的PCR-RFLP方法。基于对340条mtCOII序列的分析结果表明:所有样本共获得62个单倍型,其中单倍型17(H17)广泛分布于各种群之间,有6个单倍体型为山东种群所特有;亚洲玉米螟分化为2个遗传支系(支系Ⅰ与支系Ⅱ);2个遗传支系均在山东省发现,但以支系I为主;亚洲玉米螟各单倍体型散布于山东省各地理种群中,缺乏明显的地理分布格局。山东亚洲玉米螟总体的单倍体型多样性指数Hd为0.695,种群内单倍型多样性指数在0.333—0.889之间;总体的核酸多样性指数π为0.00424,种群内核酸多样性指数在0.00061—0.00809之间。总群体的固定系数Fst为0.79421。AMOVA分析结果表明山东亚洲玉米螟的遗传分化主要来自于2个支系之间(79.42%)。构建的亚洲玉米螟2个支系鉴别方法为其生物学与生态学的进一步研究奠定了基础。Abstract： Ostr inia furnacalis（ Guen6e）, the Asian corn borer, is one of the most important agricultural pests in China. It can damage crops during different crop growth stages, and can affect the yield and quality of maize. In addition, the pest has a wide geographic distribution and host range. With global warming and the expansion of corn acreage in recent years, the occurrence of the pest and associated damages have been increasing. Studying the genetic structure of the Asian corn borer is necessary for understanding the pest＇s evolution and migration in Shandong, a critical corn production region in China. In order to reveal the genetic diversity and structure of O. furnacalis in Shandong, China, and throughout the world, we analyzed 340 mtCOH sequences of O. furnacalis obtained both from the present study and GenBank （ updated to October 1 ~t, 2011 ）. Of the mtCOH sequences analyzed, 214 sequences were obtained from GenBank while 126 sequences were obtained during the present study. The mtCOll sequences were aligned using MEGAS. 05 and were then checked for indels and numts. Using DnaSP 5.0, a set of genetic parameters for mtCOIl were estimated including： the number of polymorphic （segregating） sites （S） ; the total number of mutations （η） ; the average number of nucleotide differences （K）; the number of haplotypes （H）; the haplotype diversity （Hd）;the nucleotide diversity （π）, defined as the average number of pairwise nucleotide differences per site; and the nucleotide diversity with Jukes and Cantor correction [ or（JC） ] for different host collections from Shandong Province. The results revealed that there were a total of 62 haplotypes, among which the haplotype H17 was the most widely distributed. Using the 62 haplotypes, a phylogenetic tree was constructed with the maximum likehood （ML） method; two clades （Clade I and Clade II ） were revealed. The populations from Shandong Province consisted of two clades, although Clade I was the dominant clade. T

关 键 词：亚洲玉米螟 遗传分化 mtCOII PCR-RFLP 山东省 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]