轴向嘧啶衍生物修饰硅酞菁的光谱性质、光动力抗癌活性及与白蛋白相互作用的研究  

Spectral Properties,Photodynamic Anticancer Activity and the Interaction with Albumin of Silicon Phthalocyanine Axially Modified by Pyrimidine Derivatives

作　　者：沈小敏[1] 蒋雄杰[1] 朱尉娇[1] 张汉辉[1] 黄剑东[1] 

机构地区：[1]福州大学化学化工学院,福建福州350108

出　　处：《光谱学与光谱分析》2013年第8期2148-2152,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(21172037);高等学校博士学科点专项科研基金项目(20113514110001);福建省自然科学基金项目(2011J01040)资助

摘　　要：研究了一种新型轴向修饰硅酞菁,即二(2-氨基-6-三氟甲基-4-嘧啶氧基)硅酞菁(SiPcF)的光物理光化学性质、离体光动力抗癌活性以及与白蛋白的相互作用。结果表明,SiPcF的Q带最大吸收峰波长686nm,摩尔吸光系数为2.3×105 mol-1.L.cm-1,荧光发射峰694nm,荧光量子产率为0.46,光敏化产生单线态氧的量子产率为0.38,是有效的1 O2光敏剂。SiPcF与牛血清白蛋白(BSA)具有较强的相互作用,两者的结合常数为4.33×105 mol.L-1,结合位点数为1。离体细胞实验表明,SiPcF具有较高的光动力抗癌活性,对人肝癌细胞HepG2的IC50值为5×10-7 mol.L-1。Photophysical photochemical properties,in vitro photodynamic anticancer activity and interaction with albumin of a new axially modified silicon phthalocyanine,i.e.di（2-amino-6-trifluoromethyl-4-pyrimidinyloxy）silicon phthalocyanine（SiPcF）,were studied in the present paper.The Q band maximum absorption of SiPcF located at 686nm with the molar absorption coefficient of 2.3×105 mol-1.L.cm-1.The fluorescence emission of SiPcF peaked at 694nm with a fluorescence quantum yield of 0.46.Its singlet oxygen quantum yield produced by photosensitization is 0.38,suggesting that SiPcF is an effective 1 O2 photosensitizer.There is strong interaction between SiPcF and bovine serum albumin（BSA）.The binding constant is 4.33×105 mol.L-1and the number of binding sites is 1.In vitro experiments show that SiPcF had a high photodynamic anticancer activity against human hepatoma cells HepG2with an IC50value down to 5×10-7 mol.L-1.

关 键 词：硅酞菁 光谱性质 光动力治疗 白蛋白 嘧啶 

分 类 号：O644[理学—物理化学]