大叶南五味子多糖的分级与纯化  

Fractional Precipitation and Purification of Kadsura marmorata Polysaccharides

作　　者：蒋红[1] 李新国 王宏军[1] 董婧婧 刘英姿[1] 侯艳杰[1] 林鹏[1] 杨嘉玉[1] 

机构地区：[1]辽宁医学院,辽宁锦州121001 [2]中牧农业连锁发展有限公司,北京100070 [3]江苏仁寿药业有限公司,江苏淮安223200

出　　处：《中国农学通报》2013年第21期206-209,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"KPS调控鸡免疫缺陷活性成分的结构解析及分子机制"(31201951);辽宁省农业科技攻关重点项目"辽宁省重大动物疫病病原变异与综合防控技术研究"(2011214001)

摘　　要：为了从大叶南五味子多糖(KPS)中获得单体,将其进行分级与纯化。用乙醇为沉淀剂对KPS进行分级,Sephadex柱色谱梯度洗脱分离各级多糖,蒽酮-硫酸比色法测定糖含量及洗脱液吸光度值。乙醇分级沉淀结果显示KPS分为Ⅰ～Ⅴ级分,糖含量分别为76.51%、78.36%、95.13%、92.41%、88.35%;Sephadex柱色谱分离结果显示,KPSⅡ、Ⅲ、Ⅴ中所含多糖分子量基本一致,为纯一多糖;KPSⅠ、Ⅳ中含有不同分子量多糖,其中KPSⅠ-1、KPSⅡ-1、KPSⅢ-1、KPSⅣ-6、KPSⅤ-1均为纯多糖。分级纯化的大叶南五味子多糖单体为其化学结构的分析奠定基础。In order to obtain the monomer of polysaccharide, Kadsura marmorata polysaccharides （KPS） was separated and purified. KPS was fractionally precipitated by the different concentration ethanol. The polysaccharide monomers were separated through Sephadex column chromatography and NaHCO3 solution gradient elution. The content of sugar and absorbance value of eluant component was detected by anthrone-vitriol colorimetric method. The results of ethanol fractionally precipitation showed that KPS was separated five fractional polysaccharides （KPSⅠ-Ⅴ）, and the content of five sugars was 76.51%, 78.36%, 95.13%, 92.41%, 88.35%, respectively. The results of separation by Sephadex column chromatography showed that KPSⅡ, KPSⅢand KPSⅤcontained the single molecular weight polysaccharide, respectively. However, KPS Ⅰ and KPS Ⅳ contained the different molecular weight polysaccharides, respectively. Those polysaccharide monomers, which included KPSⅠ-1, KPSⅡ-1, KPSⅢ-1, KPSⅣ-6 and KPSⅤ-1, were separated by Sephadex colum. This work will lay a foundation for the further research chemical structure analysis of KPS.

关 键 词：大叶南五味子 多糖 分级沉淀 分离 纯化 

分 类 号：Q539[生物学—生物化学] R284.2[医药卫生—中药学]