毛细管区带电泳测定芦荟中的有效成分  

Determination of the Active Components in Chinese Herb Aloe vera L.var. chinensis (Haw.) Berger by Capillary Zone Electrophoresis

作　　者：王德先[1] 杨更亮[1] 王琳颖 宋秀荣[1] 

机构地区：[1]河北大学化学系,河北保定071002

出　　处：《色谱》2000年第5期423-425,共3页Chinese Journal of Chromatography

基　　金：河北省自然科学基金!资助项目 (2 970 69)

摘　　要：建立了毛细管区带电泳测定中药芦荟中的有效成分芦荟甙和芦荟大黄素的分析方法。采用 2 4mmol/L的磷酸盐 (pH 1 0 85)作为缓冲溶液 ,在电压为 1 5kV和检测波长为 2 54nm的条件下进行分离实验 ,芦荟提取液中有效成分获得基线分离。定量分析表明 ,芦荟甙的质量浓度为 0 0 2 9g/L～ 1 0 0 g/L时 ,其峰面积与质量浓度呈线性关系 (r =0 997) ;芦荟大黄素的质量浓度为 5 4mg/L～ 84mg/L时 ,其峰面积与质量浓度呈线性关系 (r =0 999)。此外 ,还讨论了缓冲溶液的 pH值、有机改性剂对两种有效成分迁移行为的影响。It has been proved that the Chinese herb Aloe vera L var chinensis (Haw ) Berger as well as its active components showed many important pharmacology activities In order to find an easy and low cost method to control the quality of the herb, a CZE method for the determination of the active components aloin and aloe emodin in Aloe vera L var chinensis (Haw ) Berger was developed in this work Under the buffer conditions of 24 mmol/L phosphate (pH 10 52), applied voltage of 15 kV and detector wavelength of 254 nm, baseline separation of the active compounds in Aloe vera L var chinensis (Haw ) Berger was achieved and the active components were quantitatively analyzed The linear calibration equations of the two components are: Y=-0 140+57 2X(r =0 997) for aloin and Y=-0 393+1 08×10\+2X(r =0 999) for aloe emodin respectively In addition, the effects of buffer pH value and organic modifier on the migration behavior of the solutes were also investigated

关 键 词：毛细管区带电泳 芦荟甙 芦荟大黄素 分析 芦荟 

分 类 号：R284.1[医药卫生—中药学] O657.7[医药卫生—中医学]