不同时间间隔自体骨髓移植治疗骨不连的实验研究  

Experimental study on different time interval of autologous bone marrow transplantation in the treatment of bone nonunion

作　　者：张哲敏[1] 张国磊[1] 张延祠[1] 刘建宁[1] 

机构地区：[1]河北医科大学第三医院手外科,石家庄市050051

出　　处：《河北医药》2013年第14期2087-2090,共4页Hebei Medical Journal

摘　　要：目的探讨不同时间间隔自体骨髓移植治疗骨不连的实验研究,为临床自体骨髓移植治疗骨不连提供可靠的依据。方法选用健康新西兰白兔40只,制作骨不连模型,将所有实验动物随机分为2组:A组为20d间隔组;B组为40d间隔组。定期进行X线摄片、骨密度测定及组织学观察等,评价骨折愈合情况及骨痂质量。结果术后11d及术后21dX线摄片、骨密度测定及组织学观察等各项指标两组实验侧表现大致相同;术后31d、术后41d及术后61dX线摄片、骨密度测定及组织学观察等各项指标A组实验侧均优于B组实验侧(P<0.05)。结论自体骨髓移植可以提高骨折愈合速度及质量。适当时间间隔的骨髓重复注射能更加有效的促进骨折愈合。骨髓移植后的成骨作用主要体现在前30d,高峰值出现在10～20d。Objective To investigate the therapeutic effect of different time interval of autologous bone marrow transplantation on bone nonunion so as to provide reliable basis for treating bone nonunion in clinic.Methods Forty healthy New Zealand rabbits were selected to make bone nonunion models.All the experimental animals were randomly divided into 2 groups：group A with 20 day interval of autologous bone marrow transplantation;group B with 40 day interval of autologous bone marrow transplantation.The healing state of bone fracture and callus quality were evaluated by regular X-ray examination,bone densitometry and histological observation.Results The parameters showed by X-ray photographs,bone densitometry and the histological observation at 11 days and 24 days after the operation were roughly similar in the experimental side of both groups,however,which at 31 days,41 days and 61 days after operation in experimental side of group A were superior to those in group B（P0.05） .Conclusion Autologous bone marrow transplantation can improve healing speed and quality after bone fracture.The duplicate injection at suitable time interval can more effectively promote fracture healing.The osteogenesis after autologous bone marrow transplantation mainly appears at early 30 days,and the peak appears during 10～20 days.

关 键 词：骨髓移植 骨不连 时间间隔 动物 实验研究 

分 类 号：R457.7[医药卫生—治疗学]