光替代谷氨酸氧化酶及荧光法测定谷氨酸  

Light as a Substitute for Glutaminic Acid Oxidase and Fluorimentric Determination of Glutaminic acid

作　　者：蔡维平[1] 王细生[1] 朱庆枝[1] 王志红[1] 许金钩[1] 

机构地区：[1]厦门大学化学系

出　　处：《分析化学》2000年第10期1284-1286,共3页Chinese Journal of Analytical Chemistry

基　　金：福建省自然科学基金资助项目

摘　　要：在弱碱性介质中，紫外光的照射引起谷氨酸的光化学反应，产生Ｈ２Ｏ２和ＮＨ３，与谷氨酸氧化酶存在下的酶促反应等效。利用氯化血红素（ｈｅｍｉｎ）作为过氧化酶的替代物，催化Ｈ２Ｏ２与对羟基苯乙酸（ＨＰＡ）的偶合荧光反应，建立了间接荧光法测定谷氨酸的方法。Under the irradiation of UV-light, photochemical reaction of glutaminic acid occur and produces H2O2 and HN3 in weak alkaline medium with acetone. This reaction is equal in value for enzymatic reaction of glutaminic acid in the presence of glutaminic acid oxidase. Then, using hemin as catalyst, H2O2 produced by photochemical reaction oxidase p-hydroxyphenyl acetic acid (HPA) and causes fluorescence reaction coupled of it. Based on this result, a new fluorimetric method was developed for indirect determination of glutaminic acid.

关 键 词：光化学 荧光法 谷氨酸 测定 光替代酶 

分 类 号：O657.3[理学—分析化学]