伤寒杆菌ⅣB型菌毛诱导THP-1细胞IL-6表达的机制探讨  

Mechanism of IL-6 expression in THP-1 cells induced by type ⅣB pili of Salmonella typhi

作　　者：汪付兵[1] 涂建成[1] 

机构地区：[1]武汉大学中南医院,武汉430071

出　　处：《山东医药》2013年第23期13-16,共4页Shandong Medical Journal

基　　金：教育部博士点基金(博导类20100141110017);卫生部传染病重大专项(2009ZX10004-107)

摘　　要：目的探讨伤寒杆菌ⅣB型菌毛诱导THP-1细胞IL-6表达的机制。方法将THP-1细胞或人外周血单个核细胞PBMC分别与有ⅣB型菌毛的A21-6菌、缺失ⅣB型菌毛的pilS-菌共同孵育,ELISA法检测IL-6水平,荧光素酶报道基因分析法检测核转录因子(NF)-κB活化水平;用蛋白激酶C(PKC)活化抑制剂DECA预处THP-1细胞,然后以A21-6菌诱导,测定NF-κB活化水平和IL-6水平。结果 THP-1细胞或PBMC经有ⅣB型菌毛的A21-6菌诱导后,IL-6的产生水平以及NF-κB活化水平均显著高于相应对照组。PKC活化抑制剂DECA一定程度上能够抑制ⅣB型菌毛诱导的THP-1细胞NF-κB活化水平和IL-6水平。结论ⅣB型菌毛作为细菌一种重要致病因素,经由PKC-NF-κB通路促进THP-1细胞的IL-6表达。Objective To investigate the mechanism of IL-6 expression in THP-1 cells induced by type IV B pili of Salmonella typhi through activation of PKC-NF-κB pathway. Methods THP-I cells and peripheral blood mononuclear cell （PBMC） were incubated with the Salmonella typhi strain A21-6 or Salmonella typhi strain pilS- respectively, the level of IL-6 was detected by ELISA, the activity of NF-κB was measured by lueiferase reporter gene assay. THP-1 cells pretreated with PKC inhibitor DECA were induced by the Salmonella typhi strain A21-6, the level of IL-6 and the activity of NF-κB were detected as above. Results The type IVB piliated Salmonella typhi strain A21-6 could stimulate significantly higher expression of IL-6 and activity of NF-κB than those of typhi nonpiliated strain pilS -. The PKC inhibitor DECA reduced the expression of IL-6 and the activity of NF-κB induced by the piliated Salmonella typhi strain A21-6. Conclusion Type IV B pili as an important pathogenic bacterium, promotes the expression of IL-6 in THP-1 cells via the PKC-NF-κB pathway.

关 键 词：伤寒杆菌ⅣB型菌毛 白细胞介素6 核转录因子-κB 蛋白激酶C抑制剂DECA 

分 类 号：R392.1[医药卫生—免疫学]