爆炸伤兔脑组织水通道蛋白-4的表达变化及意义  

Expression of aquaporin-4 in the rabbit brain tissue with blast injury and its significance

作　　者：孙文江[1] 方健 刘家传[1] 张永明[1] 王金标[1] 杨艳艳[1] 王春琳[1] 汤宏[1] 朱捷[1] 

机构地区：[1]解放军105医院,合肥230031

出　　处：《山东医药》2013年第23期30-32,共3页Shandong Medical Journal

基　　金：安徽省卫生厅2006年省临床医学应用技术项目(06B64);南京军区医学科技创新重点项目(09Z009)

摘　　要：目的探讨爆炸伤兔脑组织水通道蛋白-4(AQP-4)的表达变化及意义。方法将80只新西兰大白兔随机分为外伤组(70只)及对照组(10只),外伤组制备兔脑爆炸伤模型,对照组不进行任何干预。采用免疫组化法检测AQP-4蛋白水平,RT-PCR法检测AQP-4 mRNA水平。结果与对照组比较,外伤组AQP-4蛋白及AQP-4 mRNA水平随伤后时间延长而升高,伤后3 d达最高值,7 d后开始下降(P均<0.05)。结论爆炸伤后兔脑组织AQP-4表达升高,此可能与爆炸伤引起的继发性脑水肿密切相关。Objective To investigate the expression of aquaporin-4 （AQP-4） in the rabbit brain tissue with blast inju- ry and its significance. Method Eighty New Zealand white rabbits were randomly divided into the trauma group （n = 70） and the control group （n = 10）. The rabbit brain blast injury model was prepared in the trauma group, and the control group without any intervention. The AQP-4 protein level was detected by the immunobistochemistry assay, and the AQP-4 mRNA level was detected by RT-PCR assay. Results Compared with the control group, the AQP-4 protein and AQP-4 mRNA levels in the trauma group were increased with the extension of time after injury, reached the highest value 3 d after injury, and 7 days later it began to decrease （ all P 〈 0.05 ）. Conclusion The AQP-4 expression in rabbit brain after blast injury was increased, which may be closely related to the secondary brain edema.

关 键 词：爆炸伤 水通道蛋白-4 免疫组织化学 脑水肿 

分 类 号：R651.1[医药卫生—外科学]