毛细管液相色谱柱的研制及其与质谱联用的研究  

Technique and Application of CLC-MS

作　　者：卞利萍[1] 张素艳[1] 岳贵花[1] 黄爽[1] 张祥民[1] 王洪海[2] 杨芃原[1] 

机构地区：[1]复旦大学化学系 [2]遗传工程国家重点实验室,上海200433

出　　处：《质谱学报》2000年第3期59-60,共2页Journal of Chinese Mass Spectrometry Society

摘　　要：The technique of coupling capillary liquid chromatography (CLC) with ESIMS was introduced in this article. The homemade reverse phase CLC was coupled with ESIMS to analyze the proteolysis of the BSA and TCS. Good separation efficiency is obtained in the peptide mapping and the partial sequence of peptides was deduced in the CID spectrum. Studies show that CLC-MS has high efficiency on sample separation, and has the merits of sensitivity, accuracy and speed on sample identification. CLC has the lower sample consuming and will be more widely used in the proteomics.The technique of coupling capillary liquid chromatography (CLC) with ESIMS was introduced in this article. The homemade reverse phase CLC was coupled with ESIMS to analyze the proteolysis of the BSA and TCS. Good separation efficiency is obtained in the peptide mapping and the partial sequence of peptides was deduced in the CID spectrum. Studies show that CLC-MS has high efficiency on sample separation, and has the merits of sensitivity, accuracy and speed on sample identification. CLC has the lower sample consuming and will be more widely used in the proteomics.

关 键 词：蛋白质分析 毛细管液相色谱柱 色谱-质谱法 

分 类 号：Q510.3[生物学—生物化学] O657.63[理学—分析化学]