液相色谱-串联质谱法同时测定血液中的18-甲基炔诺酮和孕酮含量  

Simultaneous Quantitative Determination of Norgestrel and Progesterone in Human Serum by High Performance Liquid Chromatography-Tandem Mass Spectrometry with Atmospheric Pressure Chemical Ionization

作　　者：吴筑平[1] 张超[1] 杨成对[1] 张新荣[1] 刘密新[1] 

机构地区：[1]清华大学化学系,北京100084

出　　处：《质谱学报》2000年第3期75-76,共2页Journal of Chinese Mass Spectrometry Society

摘　　要：A selective, reliable and rapid method for the simultaneous determination of progesterone and norgestrel concentration in human serum after taking oral contraceptive tablet has been developed using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) with atmospheric pressure chemical ionization (APCI) interface. The norethisterone was used as the internal standard. Selected transitions of m/z 313/245 for norgestrel, m/z 315/97 for progesterone and m/z 299/109 for norgestrel were monitored using multiple reaction monitoring (MRM) mode for quantitation. The assay was linear over the concentration range of 0.2-50ng/mL for norgestrel and progesterone. The lower level of quantitation in human serum was obtained at 0.2ng/mL for both norgestrel and progesterone using optimum tuning parameters. Serum concentrations of norgestrel and progesterone were determined following oral administration of norgestrel to female volunteers.A selective, reliable and rapid method for the simultaneous determination of progesterone and norgestrel concentration in human serum after taking oral contraceptive tablet has been developed using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) with atmospheric pressure chemical ionization (APCI) interface. The norethisterone was used as the internal standard. Selected transitions of m/z 313/245 for norgestrel, m/z 315/97 for progesterone and m/z 299/109 for norgestrel were monitored using multiple reaction monitoring (MRM) mode for quantitation. The assay was linear over the concentration range of 0.2-50ng/mL for norgestrel and progesterone. The lower level of quantitation in human serum was obtained at 0.2ng/mL for both norgestrel and progesterone using optimum tuning parameters. Serum concentrations of norgestrel and progesterone were determined following oral administration of norgestrel to female volunteers.

关 键 词：甲基炔诺酮 孕酮 血液 测定 色谱-质谱 

分 类 号：R979.21[医药卫生—药品] R969.1[医药卫生—药学]