工业污水对黑斑蛙蝌蚪生长发育及DNA的影响  

Effects of Industrial Effluent on Growth,Development and DNA in Tadpoles of Frog Rana nigromaculata

作　　者：高爱保[1] 苑丽霞[1] 

机构地区：[1]晋中学院生物科学与技术学院,山西晋中030600

出　　处：《水产科学》2013年第7期420-423,共4页Fisheries Science

基　　金：山西省高校科技开发项目(20101127);晋中学院博士科研启动资金资助项目(ky201106)

摘　　要：将黑斑蛙蝌蚪暴露于不同体积分数100%、20%、4%、0.8%和0%的工业污水中饲养,观察其生长发育过程,每7d记录平均体质量和平均体长及黑斑蛙的成活情况。对照组0%至100%工业污水处理后,蝌蚪的死亡率7d内,由3%升至100%;14d内,蝌蚪体长平均增长率由43.7%降至21.1%,每增高5倍体积分数,体长平均增长率降低5.6%;蝌蚪体质量平均增长率的影响由238.5%降至135.6%,每增高5倍体积分数,体质量平均增长率降低25.7%。采用单细胞凝胶电泳技术,利用CASP软件检测分析工业污水对黑斑蛙蝌蚪血细胞DNA损伤。结果表明,DNA损伤呈现剂量效应和时间效应,彗星图像拖尾增加,尾长、尾部DNA含量和Olive尾距显著增加。因此,本研究可为水质监测、水产养殖等领域提供科学依据和理论基础。Tadpoles of frog Rana nigromaculata were reared in the water containing （in volume） 100%, 20%, 4 %, 0.8% and 0% of industrial effluent and average body weight, body length and survival of the tadpoles were measured once every 7 days. The results showed that mortality rate was decreased from 3 in the control group to 100% in the 100% effluent group. Corresponding, the body length gain was descended from 43.7% to 21. 1%, at an average descended rate of 51 6% with increase in the effluent volume at a rate of 5% during 14 days. The body weight gain was decreased from 238.5% to 135.6%, at an average descended rate of 2ft. 7% with increase in the effluent volume at a rate of fi% during 14 days. The single cell gel electrophoresis （SCGE） was used to detect the breakage of DNA strand in hemocytes and the software of CASP was used to analyze the date picture. It was found that the DNA damage was shown to be of dose-effect and time-effect dependent. There was a significant increase in the levels of the damage of DNA, as well as the tail length, tail DNA, and olive tail moment （OTM）. The findings indicate that R. nigromaculata tadpoles can be used to monitor water pollution, and that the present study is a foundation for the protection of amphibians and other aquatic organisms.

关 键 词：工业污水 黑斑蛙 DNA损伤 单细胞凝胶电泳 

分 类 号：S966.32[农业科学—水产养殖]