不同栽培条件辣椒疫霉菌ITS序列同源性分析  

Homologous Analysis of Phytophora capsici ITS Sequence in Different Growing Conditions

作　　者：王辉[1] 刘长远[1] 王铎[2] 赵奎华[1] 梁春浩[1] 关天舒[1] 

机构地区：[1]辽宁省农业科学院植物保护研究所,辽宁沈阳110866 [2]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《微生物学杂志》2013年第3期35-38,共4页Journal of Microbiology

基　　金：农业部公益性行业科研专项(201003004);辽宁省科技攻关项目(2011214002);辽宁省蔬菜产业创新团队项目

摘　　要：由疫霉菌引起的辣椒疫病是辣椒生产上的主要病害。明确设施栽培辣椒土传病害和露地栽培条件下辣椒疫病疫霉病菌基因序列和同源性,为制定病害防治措施提供依据。通过病菌形态观察、ITS基因序列测定与同源性分析开展研究。由疫霉菌侵染的辣椒病害在设施和露地不同栽培形式下发生部位略有不同,但在病菌形态上表现一致,ITS基因序列同源性极高,达到了99%以上。设施土传病害和露地栽培条件下辣椒疫病菌属于同一种,均为辣椒疫霉菌(Phytophthora capsici Leonian)。Phytophthora capsici（Pc） was the main disease that caused pepper blight in pepper production.The gene sequence and homology of Pc in pepper cultivation in facilities and open plots was made certain to provide basis for the control of the disease.The study on the pathogen was developed through morphological observation,ITS sequencing and homology analysis of Pc.The infected positions on pepper by Pc had little differences in the two cultivation forms in facilities and open plots,however,the performances of the pathogenic morphology were congenial,and the ITS sequence were in extremely high homology,reached more than 99%.The pathogens that caused the disease in different cultivation forms in facilities and open plots were all the same species,Phytophthora capsici.

关 键 词：辣椒疫霉菌 栽培形式 ITS序列测定 同源性分析 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治] Q93[农业科学—植物保护]