白藜芦醇对缺氧诱导神经元凋亡的影响  

The Effects of Resveratrol on Neuronal Apoptosis Induced by Hypoxia

作　　者：万晓梅[1] 吴淑芳[2] 魏楚蓉[1] 伍吉云[1] 高红英[1] 徐悦青[1] 魏慈照[1] 李年秀[3] 王俊宜[4] 

机构地区：[1]井冈山大学神经退行性疾病与衰老研究中心 [2]井冈山大学附属医院妇产科 [3]井冈山大学附属医院质控办 [4]井冈山大学附属医院内科,江西吉安343000

出　　处：《赣南医学院学报》2013年第3期325-327,共3页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：江西省自然科学基金课题(20114BAB204015);江西省教育厅科技项目(GJJ11531);江西省教育厅教改课题(JXJG-11-15-13);2012年吉安市科技局指导性科技计划项目(编号10);井冈山大学校级课题(JZ1210)

摘　　要：目的:探讨白藜芦醇(resveratrol,RES)对缺氧(hypoxia)诱导神经元凋亡的影响。方法:原代培养小鼠皮层神经元,置于2%O2,5%CO2和93%N2,37℃培养箱中培养12 h建立缺氧模型,采用DCFH-DA和显微荧光成像系统检测神经元活性氧(Reactive oxygen species,ROS)含量;碘化丙啶(Propidium Iodide,PI)和Hoechst 33342染色检测神经元凋亡。结果:RES预处理60 min后再缺氧12 h,ROS含量和神经元凋亡率均显著低于缺氧组(P<0.01,N=3),且使用1 mM活性氧清除剂(N-acetyl-L-cysteine,NAC)也降低缺氧诱导的神经元凋亡率(P<0.01,N=6)。结论:RES抑制缺氧诱导皮层神经元凋亡,此作用可能与RES抑制缺氧所致神经元ROS增多有关。Objective：To explore the effects of resveratrol（RES） on neuronal apoptosis induced Hypoxia in cortical neurons.Methods： The present study established an hypoxic model in primary cultured cortical neurons of mice.In the hypoxia model,the level of ROS was determined using DCFH-DA and fluorescence microscopic imaging systems and apoptosis was analyzed by staining of propidium iodide（PI） and Hoechst 33342.Results： After pretreated with 25 μM RES for 60 min before hypoxia,the level of ROS and neuronal apoptosis were significantly lower than those in hypoxia cells.And pretreatment with 1 mM NAC（a ROS scavenger） can also reduce neuronal apoptosis percentage induced by hypoxia in neurons.Conclusion： The results indicated that RES could inhibit the hypoxia induced apoptosis in cortical neurons via reducing the level of ROS.

关 键 词：缺氧 白藜芦醇 凋亡 活性氧 

分 类 号：R965[医药卫生—药理学]