抗生素对不同基因型矮牵牛分化及再生的影响  

Effects of Antibiotic on Differentiation and Regeneration of Different Petunia hybride Genotypes

作　　者：陈阳[1] 袁思安[1] 赵耀[1] 吕晋慧[1] 

机构地区：[1]山西农业大学林学院,山西太谷030801

出　　处：《西南林业大学学报（自然科学）》2013年第4期107-110,共4页Journal of Southwest Forestry University：Natural Sciences

摘　　要：以矮牵牛4个品种美声晨光、黄金肉红、珊瑚红、黄金紫罗兰星条为试材,分别以卡那霉素Kan和头孢霉素Cef为选择抗生素和抑菌剂,研究不同浓度抗生素对叶片不定芽再生和试管苗生根的影响,分别筛选出适宜的抗生素浓度。结果表明:30 mg/L Kan可以抑制叶片不定芽再生,50mg/L Kan条件下,无不定芽再生。低浓度Kan即可抑制试管苗不定根再生,30 mg/L Kan条件下试管苗不再生根。550 mg/L Cef条件下,叶片不定芽再生受到抑制,而试管苗生根的适宜Cef浓度是400 mg/L Cef。The effect of Kanamycin （Kan） and Cefotaxime （Cef） with different concentrations on adventitious bud differentiation from leaflets of Petunia hybride and rooting of the tube plantlets was studied by taking 4 cuhivars, namely ＇ Meishengchenguang＇ , ＇ Huangjinrouhong＇ ,＇ Shanhuhong＇ and ＇ Huangjinziluolanxingtiao＇ as experimental materials. The appropriate antibiotic concentrations were screened. The results showed that 30 mg/L Kau could restrain adventitious bud differentiation, and no adventitious buds occurred when the concentration of Kan was 50 mg/L. Similarly, low concentration of Kan restrained the rooting of tube plantlets, while no adventitious roots took place when Karl concentration was 30 mg/L. The occurrence of adventitious buds were limited under the condition of 550 mg/L Cef, and 400 mg/L Cef was the suitable concentration for adventitious bud differentiation.

关 键 词：矮牵牛 组织培养 分化 再生 

分 类 号：S723.1[农业科学—林木遗传育种]