利用多重PCR-SSR标记鉴别三系杂交棉种子混杂和SSR位点杂合  

Distinguishment of Seeds Impurity and SSR Loci Heterozygosis in The Three Line Hybrid Cotton Using Multiplex PCR-SSR Markers

作　　者：马维军[1] 任爱民[1] 尹国[1] 张玉娟[1] 韩秋成[1] 崔明晖[1] 

机构地区：[1]邯郸市农业科学院,河北邯郸056001

出　　处：《生物技术进展》2013年第4期248-251,共4页Current Biotechnology

基　　金：国家863计划项目(2011AA10A102);河北省重大技术创新项目(11227118Z);国家转基因生物新品种培育重大专项(2011ZX08005-001-002)资助

摘　　要：杂交种纯度优劣直接决定了杂交种产量,而常规方法鉴定周期长,不能及时反映杂交种纯度,难以指导生产应用。由于种子混杂和SSR位点杂合都会造成株间带型的差异,为提高杂交种纯度鉴定的准确性,本研究从196对SSR引物中筛选到4对在双亲间表现稳定多态性的引物,根据带型特点进行组合,结果显示,利用多重PCR-SSR技术可以区分棉花杂交种种子混杂和SSR位点杂合,为杂交种快速准确鉴定提供技术支持。Hybrid seed purity decides the hybrid yield. The conventional identification method lasts long time and can not display the seed purity promptly, so not to meet the production need. Since it is difficult to distinguish the bands pattern differences of hybid lines caused by mixed seeds and SSR loci heterozygous, in order to improve the accuracy of hybrid purity identification, 4 pairs of SSR primers showing stable polymorphism in the parents were selected from 196 pairs of primers, and combined according to the characteristic of PCR banding pattern, the results showed that the multiplex PCR-SSR technology could distinguish hybrid seed impurity and SSR loci heterozygous, so as to provide technical support for rapid and accurate seed purity identification of hybrids.

关 键 词：棉花 多重PCR—SSR 种子纯度 SSR位点杂合 

分 类 号：S562[农业科学—作物学]