2,9—二甲基—1,10—菲咯啉的l—缬氨酸衍生物的合成表征及其镧(Ⅲ)配合物与DNA作用光谱研究  

Synthesis and Characterization of l-Valine Derivative of 2,9-dimethyl-1,10-Phenanthroline and DNA-Binding Spectral Study of its Lanthanum (Ⅲ) Complex

作　　者：汪中明[1] 周志芬[1] 林华宽[1] 朱守荣[1] 刘天府[1] 陈荣悌[1] 

机构地区：[1]南开大学化学系,天津300071

出　　处：《化学学报》2000年第10期1264-1269,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(29671020);天津市自然科学基金

摘　　要：以2,9-二甲基-1,10-菲咯啉为初始原料,合成了1,10-菲咯啉的l-缬氨酸衍生物——1,10-菲咯啉-2,9-二亚甲基亚氨基-(2,2＇-二异丙基)二乙酸(L).该配体经过元素分析、红外光谱、核磁共振氢谱表征.通过电子光谱,研究了La(Ⅲ)的配合物与小牛胸腺DNA的相互作用.用溴化乙锭作为荧光探针,还研究了La(Ⅲ)-L配合物与小牛胸腺DNA的相互作用的过程.作为对比,也研究了La(Ⅲ)分别与1,10-菲咯啉(Phen)、l-缬氨酸(Val)的配合物与小牛胸腺DNA的相互作用.结果表明La(Ⅲ)-L配合物与小牛胸腺DNA作用既有共价键合,又有插入作用.Starting from 2,9 - dimethyl - 1, 10 - phenanthroline, its l - valine derivative, 1, 10 -phenanthroline- 2,9 - dimethanoimino - (2,2' - diisopropyl) diacetic acid (L) was synthesized and characterized by elemental analysis, IR and 1H NMR. The protonation constants of L, the stability constants of La(Ⅲ) - L binary complexes have been determined by pH titration method at (25±0.1)°, I = 0.1 mol·L-1 NaNO3. The interaction of the lanthanum (Ⅲ) complex with calf - thymus DNA was studied by electronic spectra. Using ethidium bromide as a fluorescence probe, the binding mode of the lanthanum(Ⅲ) complex with calf-thymus DNA was studied spectroscopically. For comparison, the same measurements were carried out with La( Ⅲ) - Phen [La(Ⅲ) - 1,10 - phenanthroline complex] and La(Ⅲ) - Val [ La(Ⅲ) - l - valine complex]. The results indicate that the lanthanum(Ⅲ) complex possibly interacts with calf - thymus DNA by both intercalative and covalent binding.

关 键 词：菲咯啉 缬氨酸 DNA 光谱 镧 配合物 

分 类 号：O657.3[理学—分析化学]