新型pH敏感相分离高分子的制备及其在免疫分析中的应用  

Preparation of Novel pH Sensitive Phase Separating Polymers and Its Application in Fluorescence Immunoassay

作　　者：杨黄浩[1] 朱庆枝[1] 李东辉[1] 陈小兰 丁马太[1] 许金钩[1] 

机构地区：[1]厦门大学化学化工学院现代分析教育部重点实验室,厦门大学化学系厦门361005

出　　处：《高等学校化学学报》2000年第10期1498-1500,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金!(批准号 :2 9775 0 2 1);福建省自然科学基金!(批准号 :B9810 0 0 4)资助

摘　　要：Poly( N isopropylacrylamide methylacrylic acid)[P(NIP co MAA)], a linear water soluble pH precipitating synthetic polymer, was used as a novel separating carrier for the reactants in immunoassay. The P(NIP co MAA) precipitates out of water below a critical pH 5.6 at 37 ℃, and redissolved when the solution pH is above 6.1. This characteristic of the P(NIP co MAA) made it possible to carry out the immunochemical steps of the assay in true solution and then to quickly separate the resulting procucts from the reaction mixtures. The above approach was applied to an assay for the sandwich immunoassay of hepatitis B surface antigen(HBsAg). Sensitivity of this method was close to that of traditional ELISA using same reactants. However, the assay was much faster(assay time decreased from 100—120 min to 45 min). This method has been applied to the determination of the HBsAg levels in human blood serum with satisfactory results. This general technique may also be used for a wide variety of separation processes in addition to immunoassay, in which a specific component is to be isolated for analysis, recovery, or disposal. [WT5HZ]Poly( N isopropylacrylamide methylacrylic acid)[P(NIP co MAA)], a linear water soluble pH precipitating synthetic polymer, was used as a novel separating carrier for the reactants in immunoassay. The P(NIP co MAA) precipitates out of water below a critical pH 5.6 at 37 ℃, and redissolved when the solution pH is above 6.1. This characteristic of the P(NIP co MAA) made it possible to carry out the immunochemical steps of the assay in true solution and then to quickly separate the resulting procucts from the reaction mixtures. The above approach was applied to an assay for the sandwich immunoassay of hepatitis B surface antigen(HBsAg). Sensitivity of this method was close to that of traditional ELISA using same reactants. However, the assay was much faster(assay time decreased from 100—120 min to 45 min). This method has been applied to the determination of the HBsAg levels in human blood serum with satisfactory results. This general technique may also be used for a wide variety of separation processes in addition to immunoassay, in which a specific component is to be isolated for analysis, recovery, or disposal. [WT5HZ]

关 键 词：pH敏感相分离高分子 免疫分析 乙肝表面抗原 

分 类 号：R446.62[医药卫生—诊断学] O657.39[医药卫生—临床医学]