16型人乳头瘤病毒E6、E7蛋白和端粒酶在宫颈上皮内瘤变发展中的表达及意义  

Expression of HPV16 E6 and E7 Protein and Telomerase in Cervical Intraepithelial Neoplasia and Its Significance

作　　者：罗琼[1] 熊树华[2] 舒宽勇[2] 李景平 

机构地区：[1]香港大学深圳医院妇产科,广东深圳518053 [2]江西省妇幼保健院肿瘤科,南昌330006 [3]深圳市南山区西丽人民医院妇产科,广东深圳518055

出　　处：《南昌大学学报（医学版）》2013年第4期32-36,40,F0003,共7页Journal of Nanchang University：Medical Sciences

摘　　要：目的研究16型人乳头瘤病毒(HPV16)E6、E7蛋白和端粒酶在宫颈上皮内瘤变(CIN)和宫颈癌组织的表达,探讨其与宫颈癌前病变恶性进展间的关系,为寻找预测宫颈癌前病变转归方向的分子指标做一探索。方法采用免疫组织化学EnVision二步法检测30例原发性浸润性宫颈癌(ICC组,n=30)、CIN[CINⅠ-Ⅱ组,n=60;CINⅢ(含原位癌)组,n=30]、正常宫颈组织标本(正常组,n=10)中HPV16E6、E7蛋白和端粒酶的表达。结果 HPV16E6、E7蛋白和端粒酶在正常组、CINⅠ-Ⅱ组、CINⅢ组和ICC组中的阳性表达均呈逐级增高趋势。HPV16E6蛋白的表达:ICC组高于正常组,CINⅢ组高于正常组、CINⅠ-Ⅱ组,差异均有统计学意义(P<0.05);HPV16E7蛋白的表达:ICC组、CINⅢ组高于正常组,差异均有统计学意义(P<0.05);端粒酶的表达:ICC组高于CINⅢ组,CINⅢ组高于CINⅠ-Ⅱ组,差异均有统计学意义(P<0.05)。HPV16E6与HPV16E7蛋白、HPV16E6蛋白与端粒酶、HPV16E7蛋白与端粒酶的表达呈正相关(r=0.272,P<0.05;r=0.279,P<0.05;r=0.376,P<0.01)。结论 HPV16E6、E7蛋白和端粒酶随宫颈病变CIN的升级其阳性表达率、表达强度呈逐渐递增趋势。在宫颈癌变过程中HPV16E6、E7蛋白和端粒酶的阳性表达均呈正相关。HPV16E6、E7蛋白和端粒酶作为CIN的预后因子还尚待进一步研究。Objective To investigate the relationship of the expression of HPV16 E6 and E7 protein and telomerase in cervical intraepithelial neoplasia and cervical carcinoma to the malignant progression of cervical lesions,and to explore the molecular markers for predicting cervical lesion outcomes.Methods The expression of HPV16 E6 and E7 protein and telomerase was detected by immunohistochemistry（EnVision method） in 30 cases of invasive cervical carcinoma（ICC）,90 cases of cervical intraepithelial neoplasia（CIN） and 10 cases of normal cervices.Results The positive rates of HPV16 E6 and E7 protein and telomerase showed gradual increase in normal,CINⅠ-Ⅱ,CINⅢ and ICC groups.The expression of HPV16 E6 protein in ICC group and CINⅢ group was significantly higher than that in normal group（P＆lt;0.05）,and that in CINⅢ group was obviously higher than that in CINⅠ-Ⅱgroup（P＆lt;0.05）.The expression of HPV16 E7 protein in ICC group and CINⅢ group was significantly higher than that in normal group（P＆lt;0.05）.The expression of telomerase in CINⅢ group was significantly lower than that in ICC group but obviously higher than that in CINⅠ-Ⅱ group（P＆lt;0.05）.There was a positive correlation between HPV16 E6 expression and HPV16 E7 expression（r=0.272,P＆lt;0.05）,as well as between HPV16 E6 expression and telomerase expression（r=0.279,P＆lt;0.05）and between HPV16 E7 expression and telomerase expression（r=0.376,P＆lt;0.01）.Conclusion The positive rates and expression intensity of HPV16 E6 and E7 protein and telomerase increase gradually with the development of cervical carcinoma.There are positive correlations among HPV16 E6 expression,HPV16 E7 expression and telomerase expression in cervical carcinoma.However,further studies are needed to confirm whether HPV16 E6 and E7 protein and telomerase may be used as prognostic factors in CIN.

关 键 词：宫颈癌 宫颈上皮内瘤变 16型人乳头瘤病毒E6蛋白 16型人乳头瘤病毒E7蛋白 端粒酶 

分 类 号：R711.74[医药卫生—妇产科学]