H_2O_2介导真菌诱导子促进白桦酯醇积累  

H_2O_2 Mediated Synthesis of Betulin in Cells of Betula platyphylla Induced by Fungal Elicitor

作　　者：孙美玲[1] 李晓灿[1] 王晓东[1] 詹亚光[1] 范桂枝[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《林业科学》2013年第7期57-61,共5页Scientia Silvae Sinicae

基　　金：国家自然基金项目(31100445和31070531);中央高效基本科研业务专项资金项目(DL12CA05);黑龙江省博士后科研启动金(415288)

摘　　要：以产白桦酯醇的白桦悬浮细胞系为试材,利用药理学试验,结合高效液相色谱和荧光显微镜技术,探讨过氧化氢(H2O2)在拟茎点霉属真菌诱导子促进白桦酯醇积累中的作用。结果表明:外源H2O2降低了细胞的活力和干质量的积累量,却提高了白桦酯醇的含量。其中,1mmol·L-1H2O2处理12h,白桦酯醇的含量比对照增加89.45%,0.1mmol·L-1H2O2处理24h,白桦酯醇的含量比对照增加73.72%。真菌诱导子促进了白桦悬浮细胞中H2O2和白桦酯醇的生成,在处理24h时,分别比对照增加391.67%和185.22%。H2O2的清除剂过氧化氢酶减弱真菌诱导子对H2O2和白桦酯醇的诱导效应。由上述结果初步推断,H2O2参与了真菌诱导子诱导白桦酯醇积累的过程。The role of H2O2 in the synthesis of betulin in cells of birch （Betula platyphylla） induced by fungal elicitor was studied using suspension cell lines as the test materials. The results showed that the exogenously applied H2O2 decreased the cell viability and dry weight accumulation, however promoted the synthesis of betulin. Of them, the content of betulin was increased by 89.45% under treatment with 1 mmol·L^-1 H2O2 for 12 h, and was increased by 73.72% under treatment with 0.1 mM H2O2 for 24 h. The fungal elicitor increased the synthesis of H2O2 and betulin by 391.67% and 185.22%, respectively. H2O2 scavenger catalase （CAT） significantly decreased induction effects of the fungal elicitor in H2O2 and betulin production in the birch cells and the contents reduced by 73.10% and 35.96%. The results suggested that H2O2 participated in the fungal elicitor-induced synthesis of betulin in birch cells.

关 键 词：白桦 白桦酯醇 真菌诱导子 过氧化氢 拟茎点霉属 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]